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Q: What happen when a sample is aspirated into the flame?
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Why does when an unknown sample of a metal is placed in a flame a bright green light is released?

A green flame is due probable to copper.


when is flame test used?

to identify an unknown sample by its emission spectrum


What happen the microscope slide on the flame?

It will crack or break.


What is the sensitivity for Flame Atomic Emission Spectroscopy?

The atomic absorption spectrometer requires that the sample be atomized, broken down into individual atoms, before it is passed into the radiation beam for absorbance measurement. In flame AA, a liquid solution containing the sample is aspirated into a flame. This is achieved using a nebulizer, which mixes the sample solution with gaseous fuel and oxidant to form a uniformly mixed aerosol of the solution. There are several different phenomena which take place in the flame while the measurement is occurring. Each drop first dries to a small salt particle, then evaporates completely. The ion clusters heat further until they absorb enough energy to dissociate into free atoms in vapor state. The beam is passed through the flame and absorbance by the atomized species in the flame is measured. It should be noted that the absorbance is proportional to the concentration of ground state atoms in the flame. The flame provides a complex and reactive atmosphere. Metal atoms can undergo chemical changes, forming, for example, refractory oxides or hydroxides. Atoms can also lose electrons to form ions. Any process which converts free ground state atoms to other forms lowers the sensitivity because the ground state atoms are the absorbing species. Figure .24 shows a typical AA flame apparatus. The burner usually has a long narrow slot from which the flame emerges, and the light beam passes along the length of the slot. This allows for a longer absorbing path length, and better sensitivity. A commonly used flame is fueled with acetylene, with air for an oxidizer


Why the platinum wire is repeatedly flame sterilized in the preparation of bacterial smears?

To kill all bacteria, to ensure your sample is not contaminated.