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3,5-dinitrosalicylic acid (DNSA) reacts with sugars to form 3-amino-5-nitrosalicylic acid. This is an important reaction because the product (3-amino-5-nitrosalicylic acid) absorbs light at a very specific wavelength (540 nm) which allows for a very accurate quantification of the amount of sugar reacted. For this reason it is used in many medical and forensic testing.
Wiki User
∙ 11y ago
Wiki User
∙ 13y agoReducing sugar with disaccharide group will reduce DNSA and produce red color. Eg. Maltose
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What is the principle of DNSA method?
3,5-dinitrosalicyclic acid is an aromatic compound that react with reducing sugar to form 3-amino-5-nitrosalicyclic acid which absorbs light strongly at 540nm.
Estimation of sugars by DNSA method?
DNSA reagent appears yellow due to ts nitro gp.(chromophore) an alkaline solution of Dnsa is boiled with the reducing sugar to bbe estimated Orange brown coloration result which is read colorimetrically.
What are the methods of estimation of reducing sugar?
by comparing the colours or the amount of precipitate
What is the full form of dnsa?
3, 5- dinitrosalicylic acid is the full form of dnsa.
Does DNSA detect polysacchrides?
It detects reducing sugars. DNSA -----> 3-A, 5-N SA
What is the capital of leichstien?
vaduz
Why you are using enzyme blank and substrate blank instead of using a single blank to find out the enzyme activity?
I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the absorbance @ 540 OD. However the values are high. How can I troubleshoot high enzyme blank values in Pectinase assay ?
