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Allolactose isca sugar, isomeric with lactose, that is the true inducer of the lac operon.

An agent capable of activating specific genes. A molecule that inhibits the action of the repressor of an operon, preventing it from freely binding with the operator gene and disabling its function.

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Q: What is allolactose and how does it work?
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What are the roles of allolactose in the lac operon and tryptophan in trp operon?

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Lactose is eventually metabolized in the cell to allolactose, which binds to the lacI repressor, leading to the induction of the lac promoter (or derivatives of the lac promoters). The allolactose is also eventually metabolised. IPTG does essentially the same as allolactose, however, it does not get metabolized. Moreover, since lactose gets transported in the cell by the lacY gene product and metabolized to allolactose by the lacZ gene product, lactose cannot be used in lacZY- strains for induction. IPTG, however, can be used since it does not require the two genes.


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