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What else can I help you with?
How do they dye fireworks?
If you're talking about smoke dyes, the dye (rhodamine B, Auramine, phthalocyanine, etc...) is mixed with a 'carrier composition', usually based on Potassium chlorate and lactose or wheat flour. The burning mixture vaporises the dye to give smoke of the corresponding colour.
What is the fluorochrome staining technique for mycobacterium?
a fluorescent dye used to stain the cell walls of fungi and bacteria. The organisms then fluoresce when exposed to UV light rays. It is commonly used to visualize acid-fast bacilli (mycobacteria) in specimens. An example is acridine orange staincomsats Pharmacy (sandboy_2008@yahoo.com)
What is the method of staining for extra pulmonary tuberculosis?
The method of staining for extrapulmonary tuberculosis typically involves the use of the Ziehl-Neelsen stain, which is a specific acid-fast stain. This technique highlights Mycobacterium tuberculosis by staining the bacteria bright red against a blue or green background, allowing for visualization under a microscope. Other staining methods, such as the Auramine-rhodamine fluorescence stain, may also be employed for quicker results. These techniques are crucial for diagnosing tuberculosis in tissues or fluids affected by extrapulmonary manifestations.
What is the different staining technique in virology?
1- What_is_the_different_staining_technique_in_virology2- What are the diffrent stain in micro for virus ?
How do you grade acid fast bacilli in pus samples?
Acid-fast bacilli in pus samples are typically graded based on the number of bacilli seen under a microscope using the Ziehl-Neelsen or Auramine-rhodamine stain. The grading scale commonly used ranges from 1+ to 4+, with 1+ indicating rare bacilli and 4+ indicating numerous bacilli per high-power field. This grading helps to semi-quantitatively assess the bacterial load and guide treatment decisions for conditions like tuberculosis.
What is the chemical formula for a smoke bomb?
There isn't a "chemical formula" because they're not a single compound. A sparkler is basically finely powdered metal in a hardened paste of something that will burn packed around a thin metal wire.
Discuss the different methods of acid-fast staining?
Ziehl-Neelsen Technique. also know as the Hot stain. application of heat during staining for the greater penetration of the primary stain which is the Carbolfushin. Kinyoun techinique. also know as the Cold stain. Acid fast organisms can also be visualized by Flourescence Microscopy using specific flourescent dyes such as Auramine and Rhodamine. :D -iampol
Give the ingredients and preparation of the acid fast staining solution?
A. Ziehl-Neelsen method for acid-fast staining (6, 7)Carbolfuchsin stain: Basic fuchsin, 0.3 gEthanol, 95% (vol/vol), 10 mlPhenol, heat-melted crystals, 5 mlDistilled water, 95 mlDissolve the basic fuchsin in the ethanol; then add the phenol dissolved in the water.Mix and let stand for several days. Filter before use.Decolorizing solvent: Ethanol, 95% (vol/vol), 97 mlHydrochloric acid (concentrated), 3 mlCounterstain: Methylene blue chloride, 0.3 gDistilled water, 100 mlB. Kinyoun method for acid-fast staining (15)Kinyoun carbolfuchsin solution: Solution A. Dissolve 4 g of basic fuchsin in 20 ml of ethyl alcohol.Solution B. Dissolve 8 g of phenol (melted) in 100 ml of distilled water.Mix solutions A and B together and allow to stand for a few days.Acid-alcohol decolorizing agent: Ethanol, 95% (vol/vol), 97 mlHydrochloric acid (concentrated), 3 mlMethylene blue counterstain: Methylene blue chloride, 0.3 gDistilled water, 100 mlDissolve by shaking.C. Truant method for acid-fast staining (6, 7)Fluorescent staining reagent: Auramine O, CI 41000, 1.50 gRhodamine B, CI 749, 0.75 gGlycerol, 75 mlPhenol (heat melted crystals), 10 mlDistilled water, 50 mlMix the two dyes well with 25 ml of the water and the phenol. Add the remaining water and glycerol and mix again.Filter the resulting staining fluorescent reagent through glass wool and store at 4oC or room temperature.Decolorizing solvent: Ethanol, 70% (vol/vol), 99.5 mlHydrochloric acid (concentrated), 0.5 ml Counterstain: Potassium permanganate, 0.5 gDistilled water, 99.5 g
