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The biuret test is a chemical test used for detecting the presence of peptide bonds. In a positive test, a copper(II) ion is reduced to copper(I), which forms a complex with the nitrogens and carbons of the peptide bonds in an alkaline solution. A violet color indicates the presence of proteins. by:
Mr. Dael, Dan Rameil R.
ZSCMT II-BSMB
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∙ 15y ago
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∙ 11y agoit can be used to qualitatively tell the presence of proteins (peptide linkages) with a colour change.
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∙ 12y agoThe biuret test is a chemical test used for detecting the presence of peptide bonds
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∙ 8y agoThe Biuret solution, used for detecting peptide bonds, in made up of sodium hydroxide (NaOH), copper(II) sulfate (CuSO4), and sodium potassium tartrate.
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∙ 6y agoIn the presence of peptides a diluted solution of copper sulfate and sodium hydroxide become violet.
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∙ 12y agoIt tests for the amount of protein
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∙ 11y agoIt turns purple.
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∙ 12y agoprotein
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Is biuret test suitable to test the presence of proteins in the urine?
Yes, the biuret test will indicate the presence of peptides. If it turns violet, it is a positive result.
Does pepsin test positive in biuret test?
No
How many of these chemical groupings must be present in a molecule to give a positive biuret test?
you should have at least 2 of these,to obtain a positive result in Biuret's test. because biuret test is a test used to detect if there is peptide linkages.
Will glutamic acid give a positive biuret test?
Amino acids doesn't react with the biuret reagent.
What will you observe when the isolated beta amylase enzyme is subjected to biuret test?
When the isolated beta amylase enzyme is subjected to the biuret test, you will observe a color change from yellow to blue. This is because the biuret reagent reacts with the peptide bonds in the enzyme, causing a change in color.
Caseine in biuret test?
Casein form a purple compound in the biuret test.
Is biuret test suitable to test the presence of proteins in the urine?
Yes, the biuret test will indicate the presence of peptides. If it turns violet, it is a positive result.
Does pepsin test positive in biuret test?
No
What is the name of the test for proteins?
The biuret test
How many of these chemical groupings must be present in a molecule to give a positive biuret test?
you should have at least 2 of these,to obtain a positive result in Biuret's test. because biuret test is a test used to detect if there is peptide linkages.
What test would you run to test for the presence of a polypeptide?
Biuret test
What might Biuret reagent test in a practical real life situation?
Biuret reagent test in a practical situation tests for lipids. Biuret is used in food agents for protein amino acid chains.
How you test if foods have proteins?
The Biuret test allows one to test for proteins.
What colour is biuret before test?
Blue!!
Will glutamic acid give a positive biuret test?
Amino acids doesn't react with the biuret reagent.
Would albumin give a positive biuret test?
Hey. okay.. the biuret test is a test for peptide bonds. when CuSO4 is added to KOH, an alkaline solution, nitrogen atoms from the peptide bond forms a purple compound with the Cu2+ ions. hope this helps! read it in a text book.
What is the reagent used to test proteins?
Buiret Reagent
