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When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
Analogy for plasmid?
F-plasmid (fertility plasmid) of any conjugative bateria or Ti-plasmid (tumor-inducing) of Agrobacterium tumaefaciens.
What type of plasmid is the source of the problem with antibiotic resistance?
R plasmid.
What occurs first in the production of a recombinant plasmid?
Isolation of a plasmid from a bacterium
What is the bacterial cell called when the f factor is in plasmid form?
F Plasmid
Why pBR322 plasmid have high copy number?
I think pBR322 has a replication module from E coli plasmid colE1 ,which permits plasmid replication even when chromosome replication and cell division are inhibited by amino acid starvation and chloramphenicol, as a result, under such condition each cell accumulates several thousands copies of the plasmids up to 3000, so that one litre of bacterial culture easily yields a milligram of plasmid DNA.
How many bands were produced after pBR322 was digested with EcoR1?
pBR322 has one EcroR1 site so 1 band however if it was not fully digested you will find 2 or 3 (Linear- [cut], Supercoiled-, Round-Plasmid).
Explain the functioning of pBR322 vector with diagram?
FUNCTIONG OF pBR322
What is the advantages and disadvantages of pBR322 as a cloning vector?
pBR322 advantages is it widely used for the analysis of prokaryotic transcription and translation as well as topological changes in DNA conformation. then the disadvantage is it has only few cloning sites and the selection procedure is therefore time consuming.
WHAT IS pBR322?
pBR322 is one of the most used cloning vectors in molecular biology. Cloning vectors, best-known as plasmids, are autonomously replicating DNA units into which DNA fragments can be inserted for gene cloning. Genes taken up by these plasmids are multiplied (or cloned) as the vector replicates, to yields numbers suitable for molecular analysis. The most versatile and well-known plasmid is certainly pBR322 (in fact was one of the first ever used in gene cloning techniques) and has genetically tailored cutting sites into which DNA can be inserted without affecting plasmid self-replication. pBR322 general characteristics are: a) Size: 4.3 kb; b) Replicon: ColE1, relaxed; c) Selective markers (resistance): Amp and Tet; d) Single sites (enzymatic restriction single sites): Ava I, Pst I, BamHI, PvuII, ClaI, SalI, EcoRI, and HindIII.
What is the cloning capacity of pBR322 vector?
The cloning capacity of pBR322 vector is 1-5kb.
When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
What is the function of the Ti plasmid?
what is the function of the plasmid
Which is the plasmid that increases resistance to antibiotics?
R-plasmid
What plasmid that can degrade toluic acid?
TOL plasmid
Analogy for plasmid?
F-plasmid (fertility plasmid) of any conjugative bateria or Ti-plasmid (tumor-inducing) of Agrobacterium tumaefaciens.
A plasmid is a type of?
Plasmid is extrachromosomal DNA capable of self replication.
