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What is meant by triparental mating?

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October 22, 2008 10:03PM

Triparental mating is a method that is used to transfer plasmids from E.coli into other bacterial species. For example, to identify genes involved in utilization of methanol as a carbon source Methylobacterium the following procedure is followed:

1) create or isolate cells that cannot utilize MeOH as a carbon source (MeOH-)

- these cells