you put a solution of 5% sheeps blood and 94% Criterion and 1% injected oxygen ( to provide for semi-aerobic microbes) Do not refrigerate many people have said that it is OK to refrigerate the agar but it destroys 50% of the criterion, killing half the bacteria that should be on the plate
blood and chocolate agar plates as well as in universities laboratory Nutrient agar plates are also provided
Care must be taken if testing an organism cultured on a medium containing blood because catalase is present in red cells. If any of the blood agar is removed with the colony, a false positive reaction will occur. It is usually recommended, therefore that catalase testing be performed from a blood-free culture medium such as nutrient agar.
bacteria
You can't sterilize blood as it would hemolize. Blood is sterilized aseptically when it is purchased-meaning the animal (lets say a sheep) is killed in a certain way to ensure this happens. So the blood in your blood agar plate has already been aseptically sterilized
Agar is generally made by mixing the powdered form with varying ingredients. The powder is based on seaweed extract for nutrient agar, and nutrient agar is generally a base for most other agars - eg. Horse Blood Agar is nutrient agar with horses blood added; Choc agar has defibrinated (cooked) horses blood added.
you put a solution of 5% sheeps blood and 94% Criterion and 1% injected oxygen ( to provide for semi-aerobic microbes) Do not refrigerate many people have said that it is OK to refrigerate the agar but it destroys 50% of the criterion, killing half the bacteria that should be on the plate
No it does not pit blood agar I am curently studying Chromobcaterium violaceum and recently grew it on a sheep blood agar it did not pit it. Cv turned the agar a brown color.
Blood Agar, and trypticase soy agar grows semi fastidious
By stabbing the blood agar, the hemolysis will be easier to see and identify.
blood and chocolate agar plates as well as in universities laboratory Nutrient agar plates are also provided
Agar-agar
he is made from 2 sheeps
Care must be taken if testing an organism cultured on a medium containing blood because catalase is present in red cells. If any of the blood agar is removed with the colony, a false positive reaction will occur. It is usually recommended, therefore that catalase testing be performed from a blood-free culture medium such as nutrient agar.
sheep blood agarchocolate agar
bacteria
You can't sterilize blood as it would hemolize. Blood is sterilized aseptically when it is purchased-meaning the animal (lets say a sheep) is killed in a certain way to ensure this happens. So the blood in your blood agar plate has already been aseptically sterilized