It is used to buffer the medium (there is some phosphate buffer in medium too).
the carbonate/hydrocarbonat buffer in medium is in equilibrium with the CO2 in the incubator (usually set on 5%)
When this occurs, the membranes potenial drops, as potassium and sodium diffuse with their gradient.
Sodium chloride was needed to ensure the proteins in the cell aren't separated from the rest of the solution with the DNA.
to keep the pH opf the reaction mass towards basic.
addition of calcium chloride to a cell suspension promotes the binding of plasmid DNA to the cell surface ,which can then pass into the cell.
In soap making, sodium silicate provides buffered alkalinity that suspends soil, or stains and waste matter. It helps clean surfaces by emulsifying or counteracting soil.
act as stabilizer.
The typical glass (a sodium and calcium silicate) result from the melting of silicon dioxide, sodium carbonate and calcium carbonate; sodium carbonate ia a chemical reactant here but also help to reduce the melting point of SiO2.
It works to maintain the pH of the media in an acceptable range by acting as a buffer. see related link
FBS provide amino acids for the cell growth
Sodium is primarily stored outside the cell in the extracellular fluid. It is found in high concentrations in the fluid surrounding the cell and plays an essential role in maintaining cell function and regulating cellular processes such as osmosis and membrane potential.
Glucose is an essential component and a carbohydrate source that facilitates the growth of cells.
It is used as a cryo-protectant. DMSO prevents the formation of ice crystals and prevents cell lysis during thawing.
When this occurs, the membranes potenial drops, as potassium and sodium diffuse with their gradient.
Sodium ions play a crucial role in nerve cells by contributing to electrical signaling. When a nerve impulse is generated, sodium ions rush into the cell, leading to depolarization and the initiation of an action potential. This allows the nerve impulse to rapidly propagate along the nerve cell.
It is the medium which contains all the required enriched nutrients essential for the cells to survive in invitro condition.
The role of cloning host in RDT is to serve as the host cell under culture, in which the designed gene is subjected to produce recombinant protein.
Sodium dodecyl sulphate is a surfactant and functions as a detergent. It solubilizes the lipids present in the cell membrane and internal membrane and components of cell and allows a DNA extract free from lipids which would otherwise be contaminants in biological and biochemical assays.