0
Sodium dodecyl sulphate is a surfactant and functions as a detergent. It solubilizes the lipids present in the cell membrane and internal membrane and components of cell and allows a DNA extract free from lipids which would otherwise be contaminants in biological and biochemical assays.
Wiki User
∙ 13y ago
Add your answer:
Earn +20 pts
Role of zinc sulphate in pharmaceutical preparations?
Zinc sulphate Monohydrate is used in the pharmaceutical preparations as an antidiarrhoeal.
Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
What is the role of DEPC in RNA isolation?
It inactivate the RNase and prevent RNA to denature.
What is the purpose of isolation techniques in microbiology?
To study the specific role played by a specific microorganism in its environment.
Role of mgcl2 in DNA isolation?
mgcl2 work as a cofactor and they enhance the enzymatic reaction..
What is the role of sodium laurent sulphate in making shampoo?
Sudding agent and not good for you!
What is the role of NaCl in RNA isolation?
The role of NaCl or sodium chloride in RNA isolation is part of the denaturing process. It is often called the wash step.
What is the role of tris-hcl sodium bisulfite and sodium bicarbonate in genomic DNA isolation?
than podo
What is the function of sodium dodecyl sulphate in a DNA extraction?
The principle role in DNA isolation that sodium docdecyl sulphate (or SDS for short) provides is in the break down of the cell wall/membrane of a bacterial cell. The long hydrocarbon chain on the end of SDS is extremely hydrophobic, while its sulphate head is very hydrophilic. Because of this SDS will tend to stick itself into the cell membrane (because the inner part of the membrane is hydrophobic, and the outside is hydrophilic). However, SDS does not exactly fit into the membrane well, and will disrupt it, eventually causing the membrane to collapse. Additionally, SDS's hydrophobic tails will tend to surround integral membrane proteins in the membranes of the cells (because the proteins are largely hydrophobic as well), and because of this surround of hydrophilic SDS heads, the protein will forceably be removed from the cell membrane. Once again, this contributes to the breakdown of the cell membrane.
What is the role of sodium acetate in DNA extraction?
sodiom acetat reaction with membrane protein and cause that persipitate and help to dna isolation
Role of zinc sulphate in pharmaceutical preparations?
Zinc sulphate Monohydrate is used in the pharmaceutical preparations as an antidiarrhoeal.
What is the role of mercaptoethanol in DNA isolation?
Mercapotoethanol is used as an antioxidant. Especially for polyphenolics which become insoluble when oxidised and tend to precipitate with the DNA. Sodium sulphite (1.2 g/L) does the same thing without the stink.
Role of CTAB in DNA isolation?
It sequester carbohydrates in the solution
What is role of Cetyl trimethyl ammonium bromide in DNA isolation?
it act as as a cationic detergent for the isolation dna from the given sample
Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
What is role of BCP bromo chloropropane in RNA isolation?
for phase separation
Role of chloroform in RNA isolation?
it solubilize the lipids and protein and remove them.
