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This question has to be answered depending upon the meaning of a research project that involves proteins.
The estimation of proteins can be done to know the protein fraction of a sample collected from the field, either if the protein content will be isolated to be studied, or to remove protein fraction from the sample (sometimes the investigator does not want that proteolytic enzymes chew a target molecules or cellular structures).
Another application is when an enzymatic reaction is going to be performed to digest a particular protein in an aliquot, the protein content is crucial for stoichometric purposes.
On the other hand, if the research imply the work with nucleic acids, is very important "to inactivate" the nucleolytic enzymes present in cytoplasmic fluids from lysed cells with specific inactivators, chemical or biochemical, added in correct levels according to protein contents.
Wiki User
∙ 7y ago
Wiki User
∙ 9y agoProtein estimation is very useful when planning diet and exercise. A person needs to know what foods contain proteins so that they are sure to eat healthy.
Wiki User
∙ 11y agofolin lowry method,
biuret method,
bradfoard method
Wiki User
∙ 12y agobb b n nbn n
Wiki User
∙ 11y agolowrys method
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Is lowry's protein estimation an invasive method?
No. It is a non-invasive method
Why protein estimation is done at 660 nanometer in folin lowry method?
because folin lowry reagent has absorption maxima at 660 nm
What moves substances in and out of a cell?
Most molecules can move in and out of a cell, but different molecules have different methods of getting in- Small Non-Polar molecules can diffuse across the lipid bilayer Medium sized molecules of any kind require a specific protein channel HUGE MOLECULES require a process called endo and exocytosis
What is percent recovery in protein purification?
When you use methods such as Solvent precipitation, you precipitate a protein molecule but this protein molecule normally does not have the same structure as that of protein in a solution (for example disulfide bonds do not connect the same amino acids) and it is not easy to change this to that of original structure even when the same solution is present. The percent of recovery means the percent that these denatured protein molecules can gain the same structure that they have in the solution before precipitation.
A denatured protein may re-form to its funtional shape when returned to its normal enviorntment What does that indicate about a protein's confirmation?
a denature protein may re-form to its functional shape when returned to its normal environment. what does that indicate about a protein's conformation? Proteins fold in natural environment (water) in a way that they are stable, but a non-polar solvent provides a very different environment, so the protein has to unfold and adopt a very different shape.
What are the methods in estimation?
There are a few methods in estimation. Like framework, unknown parameters, empirical dist and substitution principle most of these methods can be used using substitution principles.
What are the methods for estimation of DNA?
diphenylamine reagent
What is an invasive method of protein estimation?
gand mara
What are the methods of estimation of reducing sugar?
by comparing the colours or the amount of precipitate
Methods for the estimation of Nacl?
For example concentration determination with a salinometer.
Why we are used sodium bicarbonate for the estimation of protein?
Sodium bicarbonate is not necessarily used in the estimation of protein. There are very many protein assays on the market, and the vast majority of them do NOT use NaHCO3. Look up Lowry, Biuret, Bradford, etc.
Is lowry's protein estimation an invasive method?
No. It is a non-invasive method
What is determining the value or amount of something by mathematical or logical methods?
There is no single word. Mathematical methods, alone, could involve analytical methods, statistical methods of estimation or numerical methods for approximation.
What is protein estimation?
here the amount of protein in a sample can be determined, using different protocols, wherein the reagent mixture( ex: FC reagent) which when added to the sample containing protein reacts with the specific amino acids giving colour, thus the amount of protein in a sample can be estimated and the data used for further protein studies.
Latest methods of screening methods for Antiepileptic drugs?
High Performance Liquid Chromatography can be used for the estimation ofmost of the antiepilleptic drugs
What has the author O Richter written?
O. Richter has written: 'Parameter estimation in ecology' -- subject(s): Ecology, Mathematical models, Parameter estimation, Statistical methods
What percent of snake venom is protein?
Average protein estimation in snake venom is from 49.8 to 96.4% . the age of the snake may affect the percentage of protein content found in the venom
