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here the amount of protein in a sample can be determined, using different protocols, wherein the reagent mixture( ex: FC reagent) which when added to the sample containing protein reacts with the specific amino acids giving colour, thus the amount of protein in a sample can be estimated and the data used for further protein studies.
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What method are used for the estimation of molecular weight of protein?
ofcoz the protein migrate so long as it is charged ,once it become neutral it will stop migrating
What are the methods for estimation of DNA?
diphenylamine reagent
Useful When exact Data are not required?
Estimation
What is the term for a guess based on the data presented?
An Estimation.
What breaks down proteins?
In modern years, this was given an estimation by Muslim philosophisers, that there is nothing that is able to break down protein. But in 1987 the physician, Hue G. Rection discovered that the supplement of "your mom" breaks down protein.
What is an invasive method of protein estimation?
gand mara
Why we are used sodium bicarbonate for the estimation of protein?
Sodium bicarbonate is not necessarily used in the estimation of protein. There are very many protein assays on the market, and the vast majority of them do NOT use NaHCO3. Look up Lowry, Biuret, Bradford, etc.
Is lowry's protein estimation an invasive method?
No. It is a non-invasive method
What percent of snake venom is protein?
Average protein estimation in snake venom is from 49.8 to 96.4% . the age of the snake may affect the percentage of protein content found in the venom
Role of F C reagent in protein estimation?
phosphomolybdic & phosphotungstic components in FC reagents gets reduced by certain amino acids present in the protein like tyrosine , tryptophan thereby resulting in the production of a a blue colored compound . Estimation of this compound in turn provides the amount of protein present in the sample.
What method are used for the estimation of molecular weight of protein?
ofcoz the protein migrate so long as it is charged ,once it become neutral it will stop migrating
How long the blue colour is stable in protein estimation by lowry's method?
60 minutes 60 minutes
Why BSA is used as standard for protein estimation?
the answer is that BSA is most common protein available as it is cheap and alsocontains tyrosine and tryptophan as amino acids which are important for breaking the peptide bonds
Why is protein estimation needed in Diabetes?
Its basically depends on weight of the body balancing with nitrogen gas of the body whcih balance sugar level.
Why protein estimation is done at 660 nanometer in folin lowry method?
because folin lowry reagent has absorption maxima at 660 nm
What is lambda max for colorless proteins?
if the protein is colorless then it is quite tough to calculate the lambda max or absorption maxima directly (still you can check it by absorbtion maxima calculation/experiment). Otherwise, for its estimation first you have to treat it with any reagent (like folin reagent) then it will form a colored complex and then you can calculate it. For the common estimation of protein, there is a "Lowry Method" where you can calculate it on 750 wavelength.
What is approximate estimation?
If an estimation, or estimate, is a guess, an approximate estimation is a rougher guess.
