0
dna binding protein binds the 2 anti parallel strands of dna together
Wiki User
∙ 12y ago
Add your answer:
Earn +20 pts
What are three techniques used to determine the specific DNA binding site of a DNA-binding protein?
Well to determine the binding site of the protein, you would need to determine the structure of the protein. one of the techniques used is NMR (nuclear magnetic resonance..i think lol).
How does EMSA electromobility shift assay measure whether the protein bends DNA?
EMSA does not measure if protein bends to DNA. It does measure what proteins bind to a specific region of DNA (usually a promoter region). You can use a supershift to determine exactly what protein is binding to the specific DNA region.
Is DNA in archea?
actual histone particles are not present in archaebacteria they have some other type of dna binding protein
What is the correct pathway of information flow in the cell?
Information is picked up from the dendrites and then transfered to the soma (which is through action potentials) and then transfered to the axon which then goes to the presynaptic terminals that sends the information to the next neuron which will then repeat this flow of information.
What is Electromobility shift assay?
One method for detecting DNA-protein interaction. EMSA rely on the ability of proteins to bind radiolabels DNA fragments in vitro. Once binding occurs, DNA-protein complexes can be separated from unbound DNA fragments by nondenaturing polyacrylamide gel electroforesis. In general the larger the protein or protein complex bound to the DNA, the greater the extend of retardation of mobility of the radiolabeled DNA fragment. The resulting protein-DNA complexes are visualized by autoradiography.Methods in muscle biology, Charles P. Emerson, H. Lee Sweeney.
What is ssb?
In genetics, SSB stands for single-stranded DNA-binding protein. These are proteins that bind to single-stranded areas of DNA to prevent early binding and to protect the DNA from being digested by nucleases.
What protein does DNA polymerase remove as it adds nucleotides?
When the replication fork is moving towards DNA double strand in the direction 5'- 3', a "Single-strand Binding Protein" (or SSB) -a dnaB gene product- must be removed in order to allow DNA polymerase to add the following nucleotide.
When was RNA-binding protein database created?
RNA-binding protein database was created in 2010.
What exhibits fourth level structure?
Protein often exhibit fourth level or quaternary structure. The dimer, is the simplest form of fourth level structures is called a dimer, which is seen in DNA binding protein.
In gene expression and protein synthesis information flows from?
DNA --> RNA --> Proteins -----------------------------------------That simple.
What is the bacteriophage used by hershy and chase consisted of two types of molecules?
protein and DNA
What holds DNA strands apart during replication?
What prevents the wrong nucleotide from being added to the new strand during DNA replication? DNA polymerase 3 and DNA polymerase 1 can become what is known as exonucleases. an exonuclease can go back and "proofread" the replicated DNA and if there is a mistake, then everything beyond that incorrect nucleotide is removed and the DNA polymerase 3 will re-replicate from the bad point on. the protein p53 holds the cell in the G1 and S phase of replication which allows more time for proof reading the replicated DNA
