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Separate plasma proteins by electrical charge?
electrophoresis
Electrophoresis is used to sort DNA by?
Electric charge
Why was it important to stop the gel electrophoresis before the dye ran off the edge of the gel?
You would lose your DNA.
Which process is used to separate molecules by movement due to size and electrical charge?
gel electrophoresis
What is the purpose of a stain in electrophoresis?
I think the Question should be " What is the use of a dye in electrophoresis?" to be more specific, The normal dyes that are incorporated into the sample loading buffer in case of agarose gel is bromophenol blue and xylene cyanol.These are used to track the movement of the sample, so that we can stop the electrophoresis when the dye front is nearly 80%(can vary depending on the molecular weight of the sample) away from the well. In case of polyacrylamide gel electrophoresis the dye has many functions: gives density to the sample so that it sits properly in the well. SDS binds to the amino acid and makes it anionic, so that the movement of the sample is based on molecular weight, if not the charge of the protein will also influence the movement. it also helps us to track the movement of the sample. DTT or mercaptoethanol helps in the breaking of cysteine bonds.. That's it.. But the question is too broad and can be specific!!!!!!!!!1
Separate plasma proteins by electrical charge?
electrophoresis
What is the principle of electrophoresis?
Electrophoresis works on the principle of migration of charged particles (toward their opposite charge) in the presence of electric field.
Why does sickle cell hemoglobin migrate slower than normal hemoglobin during electrophoresis?
because of the change of AA- in normal cell- from Glutamic acid (negativity charged) to Valine (uncharged) -in sickle cell- the charge will be missing in the sickle cell that why the electrophoresis will become slower because of the missing charge
Why does sickle cell hemoglobin migrate slower than normal hemoglobin during gel electrophoresis?
because of the change of AA- in normal cell- from Glutamic acid (negativity charged) to Valine (uncharged) -in sickle cell- the charge will be missing in the sickle cell that why the electrophoresis will become slower because of the missing charge
Is electrophoresis part of biotechnology?
Electrophoresis is used to separate molecules based on size and charge. Since biotechnology depends on knowing what you are working with, electrophoresis of proteins, DNA and RNA is a tool used by biotechnologists.
For what purpose might one use capillary electrophoresis?
Capillary electrophoresis is a technique used in laboratories to separate molecules based on their charge in order to study and analyze them. Capillary electrophoresis uses an electric charge to force the movement of molecules since each molecule will go a varying distance based on the weight of the molecule and their charge. Some areas of study that use capillary electrophoresis include DNA sequencing and pharmaceutical analysis.
Method of separating out plasma proteins by electrical charge?
Electrophoresis
What is th blue dye added to the sample before the electrophoresis is performed?
In biochemistry labs, the traditional answer for a protein gel (polacrylamide gel electrophoresis) is bromphenol blue. For a DNA gel (agarose gel electrophoresis), traditionally the same dark blue dye bromphenol blue was combined with the lighter, slower migrating blue dye xylene cyanol. Oftentimes nowwe only use the bromphenol blue, or even substitute for it with Orange G, which is a UV-transparent dye that more easily enables the visualization of smaller molecular weight nucleic acids that migrate in the same region.
What separates molecules by movement due to size and electrical charge?
gel electrophoresis
What are the name of separating techniques involving electric charge on molecules?
Gel electrophoresis.
Electrophoresis is used to sort DNA by?
Electric charge
Why was it important to stop the gel electrophoresis before the dye ran off the edge of the gel?
You would lose your DNA.
