there is only difference in gram positive and negative is of cell wall as :
The Gram positive cell wall
The Gram positive cell wall is characterised by the presence of a very thick peptidoglycan layer, which is responsible for the retention of the crystal violet dyes during the Gram staining procedure. It is found exclusively in organisms belonging to the Actinobacteria (or high %G+C Gram positive organisms) and the Firmicutes (or low %G+C Gram positive organisms). Bacteria within the Deinococcus-Thermus group may also exhibit Gram positive staining behaviour but contain some cell wall structures typical of Gram negative organisms. Imbedded in the Gram positive cell wall are polyalcohols called teichoic acids, some of which are lipid-linked to form lipoteichoic acids. Because lipoteichoic acids are covalently linked to lipids within the cytoplasmic membrane they are responsible for linking the peptidoglycan to the cytoplasmic membrane. Teichoic acids give the Gram positive cell wall an overall negative charge due to the presence of phosphodiester bonds between teichoic acid monomers.
The Gram negative cell wall
Unlike the Gram positive cell wall, the Gram negative cell wall contains a thin peptidoglycan layer adjacent to the cytoplasmic membrane, which is responsible for the cell wall's inability to retain the crystal violet stain upon decolourisation with ethanol during Gram staining. In addition to the peptidoglycan layer the Gram negative cell wall also contains an additional outer membrane composed by phospholipids and lipopolysaccharides which face into the external environment. The highly charged nature of lipopolysaccharides confer an overall negative charge to the Gram negative cell wall. The chemical structure of the outer membrane lipopolysaccharides is often unique to specific bacterial strains (i.e. sub-species) and is responsible for many of the antigenic properties of these strains.
As a phospholipid bilayer, the lipid portion of the outer membrane is largely impermeable to all charged molecules. However, channels called porins are present in the outer membrane that allow for passive transport of many ions, sugars and amino acids across the outer membrane. These molecules are therefore present in the periplasm, the region between the plasma membrane and outer membrane. The periplasm contains the peptidoglycan layer and many proteins responsible for substrate binding or hydrolysis and reception of extracellular signals. The periplasm is thought to exist as a gel-like state rather than a liquid due to the high concentration of proteins and peptidoglycan found within it. Because of its location between the cytoplasmic and outer membranes, signals received and substrates bound are available to be transported across the cytoplasmic membrane using transport and signalling proteins imbedded there.
They have different cell walls.
Gram-negative bacteria have a thinner peptidoglycan layer in their cell walls compared to gram-positive bacteria. Additionally, gram-negative bacteria have an outer membrane that contains lipopolysaccharides, which is absent in gram-positive bacteria.
Gram positive bacteria cell walls have a thick layer of Peptidoglycan and no periplasmic space. Gram negative bacteria cell walls have inner and outter cytoplasmic membranes with a periplasmic space in between. these also have a thin layer of Peptidoglycan. The outter cytoplasmic membrane of gram negative bacteria contains lipopolysaccharides.
Candida albicans is a yeast and belongs to the fungal kingdom, so it does not have a gram classification like bacteria. Gram staining is a technique used to differentiate between gram-positive and gram-negative bacteria based on the composition of their cell walls.
The cell walls are the difference. Gram stain will be positive, as the cell wall is thicker. Gram-positive bacteria retain the (crystal violet) dye. Then a decolorizer will be added and then the pink counterstain. This will give all gram-negative bacteria a red or pink coloring. All the steps are done for all cultures being Gram stained and when they grow, the color will be seen as well as the shape.
If the Gram stain is properly done, and based on color alone: - you CANNOT distinguish between Staphylococcus (positive/purple) and Streptococcus (positive/purple) - you CAN distinguish between Staphylococcus (positive/purple) and Neisseria (negative/red) - you CANNOT distinguish between Escherichia (negative/red) and Proteus (negative/red) - you CAN distinguish between Escherichia (negative/red) and Bacillus (positive/purple) Gram positives will stain purple because of the retention of the dye (crystal violet) in their thick peptidoglycan cell walls. On the other hand, Gram negatives have a thin cell wall and cannot retain the purple stain, so when they are counterstained, they will appear red
Gram positive bacteria stain purple with the Gram stain. This is because of the fact that they contain a thick layer of murein in their cell wall, which takes up the stain very well. Gram negative bacteria, however, do not display the thick layer of peptidoglycan on their outer surface. Therefore, they stain red with the counter stain.
Archaebacteria are neither gram positive nor gram negative because they do not have peptidoglycan in their cell walls like bacteria. Instead, they have unique cell wall structures that make them distinct from both gram positive and gram negative bacteria.
A Gram negative pathogen. This means it has two thinner petidoglycan cell walls.
Hepatitis B virus is not a gram-negative or gram-positive bacterium because it is a virus, not a bacteria. Viruses do not have cell walls like bacteria, so they are not classified as gram-negative or gram-positive.
Mycolic acid is primarily found in the cell walls of mycobacteria, which are a type of bacteria that include the causative agents of tuberculosis and leprosy. It is not typically found in the cell walls of other bacteria, including those classified as gram-positive or gram-negative.
The two different types of cell walls found in eubacteria are gram-positive and gram-negative cell walls. Gram-positive cell walls have a thick layer of peptidoglycan, while gram-negative cell walls have a thinner layer of peptidoglycan surrounded by an outer membrane.