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What is the difference between a sterile broth and contaminated broth can be?
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What would have happened is Pasteur had tipped on of his flasks so that the broth in the flask came into contact with the curve of the neck?
If the pasteur tipped on of his flasks so that the broth came into contact with the curve of the neck, the sterile broth soon became contaminated with microorganisms which were trapped in the curve.
What is Pasteurs experiment?
Showed that sterile broth remained free of microorganisms until exposed to air.
Can you preserve or freeze a bacterial isolate in a normal freezer by just growing them in a broth with glycerol?
Add the sterile glycerol after growing them in broth. However, -80 freezer preserves them for longer than normal freezer.
How many liquid ounces are in 10.75 ounces of condensed broth?
10.75Ounces is ounces. "condensed" makes no difference.
Is broth has agar?
no, broth stands for liquid and agar is used as solidifying agent so broth is not containig agar.
What would have happened is Pasteur had tipped on of his flasks so that the broth in the flask came into contact with the curve of the neck?
If the pasteur tipped on of his flasks so that the broth came into contact with the curve of the neck, the sterile broth soon became contaminated with microorganisms which were trapped in the curve.
What is the difference between 'broth and stew' in definition?
ok broth is a more watery like substance while stew is very rich and thick
What is Pasteurs experiment?
Showed that sterile broth remained free of microorganisms until exposed to air.
What is low sodium broth?
Many brands of beef or chicken broth will market a “standard” or “regular” broth, as well as a low-sodium version. The biggest and most obvious difference between the two options is that the reduced-sodium broth has less sodium in it than the “regular” broth. AustralianBoneBroth
Who discovered the falsified spontaneous generation?
Louis Pasteur is credited with discovering the falsity of spontaneous generation through his experiments with sterilized broth in swan-necked flasks. His results showed that microbial growth only occurred when the flask was exposed to air, debunking the idea that life could spontaneously arise from non-living matter.
How do you determine if a broth is sterile?
Check for any non-granular sediments if the broth was stationary.Check for turbidity in the broth.Turbidity can be checked by tilting the flask towards light and slowly rotating it while keeping an eye on any difference in light transmittance/color/texture/threads/anything that is different from the rest of the broth!If not sure, autoclave it again!!P.S. Turbidity and sediments can only be visible if there was ample incubation, it will NOT be visible at the moment you make it!!Other advanced way can be in the use of spectroscopy. Take fresh broth as blank and suspected broth as sample and check for optical densities. If the suspect one has a greater turbidity than the fresh one then probably your broth is contaminated.P.S. Right wavelength is the key! It should be set according to the color of your broth!Good luck!
Can you preserve or freeze a bacterial isolate in a normal freezer by just growing them in a broth with glycerol?
Add the sterile glycerol after growing them in broth. However, -80 freezer preserves them for longer than normal freezer.
What is ratio between blood and broth in blood broth?
blood should be 5% by solution
Why does nutrient broth go cloudy?
Nutrient broth becoming cloudy in appearance is usually a good indication that one or more cultures of bacteria or fungi has grown in the broth. This can be confirmed by viewing a sample of the broth under a simple light microscope, or by streaking a sample onto nutrient agar and incubating it to see if colonies become visible (usually within a few days).
What is the difference between tomato soup and tomato bisque soup?
Tomato bisque is more of a paste, where soup is more watery, depending how thick the broth is.
If a recipe calls for chicken stock can you use chicken broth instead?
Yes. The only difference is the way the broth is simmered. When making broth you use the whole chicken whereas stock you use the bone. The only time it would make a difference is when you are using it to degalze a pan. Unlike broth, stock will bind with the dripping to create a sauce.
What is the difference between a nutrient broth and media?
there's an excellent explanation here http://forums.biotechinstitute.org/showthread.php?t=93
