it can change the shape of enzymes & active site so the enzyme is unable to work
pH and Temperature both impact the enzyme's function.
The pH is varied to effect, by its affect, this test.
Different enzymes work best at different pH. This is refered to as the ideal pH for the enzyme. For example, the digestive enzyme trypsin works best at an acidic pH while alkaline phosphatase works best at a basic pH. Therefore, enzyme activity varies with pH and this variation depends on the enzyme being studied
temperature,pH and substrate concentration
Heating pure water the pH decrease.
pH and Temperature both impact the enzyme's function.
pH
The pH is varied to effect, by its affect, this test.
Different enzymes work best at different pH. This is refered to as the ideal pH for the enzyme. For example, the digestive enzyme trypsin works best at an acidic pH while alkaline phosphatase works best at a basic pH. Therefore, enzyme activity varies with pH and this variation depends on the enzyme being studied
excess cofactor
nothing
temperature and pH
temperature,pH and substrate concentration
Heating pure water the pH decrease.
All enxymes work at an optimum pH. Changes in pH levels could change the rate at which the enzymes work by denaturing them.
Physiological conditions, such as correct pH and heat in which the enzyme is able to function correctly, are required.
temperature, pH, concentration of enzymes, and concentration of substrates.