0
What is the enzyme responsible for conversion of negatively supercoiled DNA into relaxed circular DNA?
Wiki User
∙ 12y ago
Topoisomerase 1
Wiki User
∙ 12y ago
Add your answer:
Earn +20 pts
How does ethidium bromide interact with double stranded DNA Does it increase or remove supercoiling?
when ethidium ion intercalates between two dna base pairs in a circular dna it causes the dna to unwind by 26 degrees, thereby decreasing twist and increasing writhe. in a circular dna which is negatively supercoiled, if ethidium is added it will become relaxed and if more ethidium is added dna becomes positively supercoiled
What bio molecule present in mitochondria allows it to reproduce independantly of the cell?
DNA is responsible for reproduction.Mitochondria have circular DNA.
Does DNA have mitochondria?
The mitochondria have its own DNA. They are referred to as the powerhouse of a cell and are responsible for converting the energy from food into a form that cells can use.
Which force is responsible for making planets and satellites travel in near circular orbits?
Gravity and inertia. Inertia keeps the planets moving while the gravity of the sun keeps the planets drawn to orbit in ellipses.
Why E coli chromosome is circular?
Circular. All bacterial chromosomes are circilar.
How does ethidium bromide interact with double stranded DNA Does it increase or remove supercoiling?
when ethidium ion intercalates between two dna base pairs in a circular dna it causes the dna to unwind by 26 degrees, thereby decreasing twist and increasing writhe. in a circular dna which is negatively supercoiled, if ethidium is added it will become relaxed and if more ethidium is added dna becomes positively supercoiled
Why are two confirmations of plasmids seen when run on a gel when naturally its always negatively super-coiled?
During the isolation of plasmid it is possible that the shearing forces may cause the DNA to break opening the DNA molecule into linear DNA rather than circular supercoiled DNA. The linear DNA moves slower than the circular DNA. Thus two bands might be seen.
What muscles are responsible for pupil construction and dilation?
circular muscles
What is the function of solution 2 in plasmid isolation?
This solution contains the detergent sodium dodecyl sulfate (SDS) which dissolves the cell membrane and denatures proteins. The solution is very alkaline (pH > 12) due to the presence of sodium hydroxide. The high pH aids in denaturing proteins and causes the cleavage of the phosphate bonds in RNA. This eliminates interference from high molecular weight RNA during the plasmid purification. Under highly alkaline conditions, the two strands in non-supercoiled DNA (linear fragments of chromosomal DNA, relaxed and nicked circular DNA) separate and are partially removed from solution. However, this does not occur with supercoiled forms of plasmid DNA because the two strands are intertwined and entangled in a way that prevents them from coming apart. Therefore, supercoiled plasmid remains free in solution.
What bio molecule present in mitochondria allows it to reproduce independantly of the cell?
DNA is responsible for reproduction.Mitochondria have circular DNA.
What is .000025 circular inches to circular mil?
.000025 circular inches = 25 circular mils
What is a formula that depends on its own value?
A circular reference. It will cause an error.A circular reference. It will cause an error.A circular reference. It will cause an error.A circular reference. It will cause an error.A circular reference. It will cause an error.A circular reference. It will cause an error.A circular reference. It will cause an error.A circular reference. It will cause an error.A circular reference. It will cause an error.A circular reference. It will cause an error.A circular reference. It will cause an error.
What is circular motion?
Circular Motion -a motion along a circular path or the motion of an object in a circular Example -blades of a ceiling fan when the fan is switched on. or The motion of body along the circular path is called circular motion
What is the structure of the plasmids?
A plasmid is a double stranded circular DNA
When an object moves at a constant speed in a circular path it is said to be in what motion?
circular
Does DNA have mitochondria?
The mitochondria have its own DNA. They are referred to as the powerhouse of a cell and are responsible for converting the energy from food into a form that cells can use.
How do you spell circular in spanish?
The same - circular
