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What is the function of QIAzol Lysis Reagent during RNA extraction?
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What are the different methods of DNA and RNA extraction?
DNA extraction is done by three methods: * Organic extraction * inorganic extraction * solid state method In organic extraction, phenol and chloroform are used to create on organic phase in which cells are lysed and DNA is freed. The DNA remains in the aqueous phase. Ethyl alcohol is used to precipitate the DNA. In the inroganic methos, NaCl and EDTA are used for cell lysis. Following this, an approach similar to the organic method is followed. In solid state extraction, DNA is first precipitated in the presence of high slat and low pH conditions. The precipitated DNA is then adsorbed on to a filter membrane surface.
What is Buffer ATL?
Buffer ATL is a proprietary chemical used for DNA extraction and purification. It's used to induce lysis of cell tissues in order to release and expose the cell's DNA during the beginning stages of of the extraction process. I think the letters "ATL" stand for "Animal Tissue Lysis." It's part of the DNeasy protocol from Qiagen. I use this every day during my extraction and purification of DNA from various snake species.
What are the precaution while conducting DNA extraction?
Perhaps the most basic precaution is to make sure that the warm water bath that will be used has a temperature that doesn't exceed 90C so as to denature only the protein component of crude extract and not the DNA which is the target isolate.
The tuberosity is fractured during extraction of maxillary third molar.The tooth with the tuberosity remains attached to the surrounding soft tissue.what will you do?
leave the tuberosity in place and stabilise it
What is the function of peptidyl transferase during translation?
to form peptde links
What the function of edta during protein extraction?
glycerol increases the stabilization of the protein by decreasing the surface tension of water
What is function of Buffer AP1 in DNA extraction?
The buffer AP1 is vital in DNA extraction as it acts as a cleanser to break up the lipids surrounding the cellular membrane. The buffer also maintains the right environment for the DNA so it is not damaged during the extraction process.
Why is sodium carbonate added during solvent extraction?
It is usually added during an extraction in order to make the solution alkaline. Often times, this aids in the efficiency of the extraction.
What purpose does sodium chloride serve during extraction of caffeine?
Sodium chloride improve the yield of caffeine extraction from water during the process of decaffeinization.
What is the purpose of Trichloroacetic acid in DNA extraction?
Trichloroacetic acid is used for precipitation of the DNA during its extraction.
How do you decolourise schiffs reagent?
With periodic acid during the steps of staining slides.
Why grind the liver in a DNA extraction lab?
the purpose of grinding any substance during dna extraction is cell loosening.
Which of the two layers that are formed during the extraction is dichloromethane?
aqueous layer
Who was on the throne of Britain during the romantic period?
King George III and his son the prince reagent
In industry cyanide are widely used during?
The extraction of gold and in electroplating of metals.
In industry cyanid compounds are widely used during?
The extraction of gold and in electroplating of metals.
Can a lingual nerve damaged during wisdom tooth extraction be repaired?
It depends on how badly damaged the nerve is. If it is completely severed, the likelihood of restoration of complete function is low. If the nerve is simply bumped or touched, it will usually heal on its own within 12 months. The most frequent nerve damaged during wisdom tooth extraction is actually the mandibular nerve, which runs through the lower jaw and provides sensation to all the lower teeth on one side.
