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The methylene blue reduction test is based on the fact that the color imparted to milk by the addition of a dye such as methylene blue will disappear more or less quickly. The removal of the oxygen from milk and the formation of reducing substances during bacterial metabolism causes the color to disappear. The agencies responsible for the oxygen consumption are the bacteria. Though certain species of bacteria have considerably more influence than others, it is generally assumed that the greater the number of bacteria in milk, the quicker will the oxygen be consumed, and in turn the sooner will the color disappear. Thus, the time of reduction is taken as a measure of the number of organisms in milk although actually it is likely that it is more truly a measure of the total metabolic reactions proceeding at the cell surface of the bacteria.

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13y ago
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12y ago

It is necessary because it consumes very less time and tells us about the quality of a milk sample. thought the best method is a plate count method but it needs minimum 24-48 hours to give results and to tell us about the bacterial content of the milk. methylene blue reduction test gives result in maximum 6-8 hours and tells about the active metabolizing bacteria present in the milk. this is commonly done in milk and food industries when the industry receives thousands of liter milk from milkmen.

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9y ago

By adding a dye to the milk like methylene blue will disappear less or more quickly. This depends on the quality of the sample of milk.

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Q: Methylene blue reduction test of milk principle?
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