salt binds to molecules to keep it from clumping
The salt neutralizes the DNA's negative charge with its positive charge while the DNA precipitates.
removal of polyphenols
phenol helps to remove non polar proteins and lipids from the solution
phenol helps to remove non polar proteins and lipids from the solution
how to make sodium citrate in 10% ethanol for DNA extraction
The salt neutralizes the DNA's negative charge with its positive charge while the DNA precipitates.
To achieve precipitation DNA.
chelating Mg2+
It solubalize lipids and protiens to remove them from DNA
DTT is the reducing agent for thiolated DNA
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
removal of polyphenols
ammonium acetae use to percipitate DNA from water.
to remove excess phenol from DNA to remove excess phenol from DNA
Triton X-100 is used as a lysis buffer for DNA separation.
The buffer AP1 is vital in DNA extraction as it acts as a cleanser to break up the lipids surrounding the cellular membrane. The buffer also maintains the right environment for the DNA so it is not damaged during the extraction process.
EtBr interculates into DNA and when exposed to UV light, it causeses DNA to nick and therefore uncoil