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What is the different reaction of Gram-negative and Gram-positive bacteria to Gram's stain testing?
Gram-positive bacteria which take up the stain turn purple, while Gram-negative bacteria which do not take up the stain turn red.
Are acid fast bacteria gram positive or negative?
it is positive
Difference between the gram stain ans acid fast stain reactions of E. coli?
Gram staining is useful in separating bacteria into two groups: Gram positive or Gram negative. They are separated into these groups based on their cell wall structure. Gram positive bacteria contain a thick layer of peptidoglycan in their cell walls, while Gram negative bacteria contain a very small layer of peptidoglycan (15% or less of what Gram positive cell walls contain). A primary stain is added, such as Crystal Violet, that will stain all of the bacteria. Then, a mordant (such as iodine and potassium iodide) is added to form a complex between the peptidoglycan and the stain, which will make the cell more resistant to decolorization. Then, a decolorizing agent is added, which will remove the primary stain from Gram negative bacteria, but will cause the cell walls in Gram positive bacteria to dehydrate, and therefore, they will retain the primary stain. Finally, a counterstain (typically safranin) is added to distinguish Gram positive from Gram negative. Gram positive cells will be purple, and Gram negative cells will be red if Crystal Violet and Safranin are used.Acid-fast staining is entirely different. Is is used to detect species of bacteria in the genera Mycobacteria and Nocardia. These bacteria are resistant to typical staining procedures, such as Gram staining, due to a thick, waxy lipid layer in the cell wall composed of mycolic acid. Heating of the bacteria with a very strong stain such as carbol-fuchsin is necessary to "melt" this lipid layer, and force the stain through the cell wall. Once the bacteria has cooled, they will be incredibly resistant to decolorization. Non-acid fast bacteria do not contain this mycolic acid layer, and therefore, they will decolorize much easier, and are then stained with a counterstain to distinguish Acid-Fast bacteria from Non-Acid-Fast bacteria.
Is mycobacterium phlei gram positive or negative?
Although mycoplasma lack a cell wall and therefore test gram negative, they are considered to be descendents of 'nonsporulating and endospore forming gram-positive bacteria' (Madigan et al., 2009), such as Lactobacilli, Bacilli and Streptococci, (Dandekar et al., 2002), which have lost their cell wall. Mycoplasmas are therfore classed as gram- positive bacteria. This is supported by ribosomal RNA and DNA analysis.
What are examples of acid-fast organisms?
Two acid-fast bacteria that are pathogenic are:1. Mycobacterium tuberculosis2. Mycobacterium avium- Microbioman
What gram reaction do you expect from acid-fast bacteria?
Gram positive
What is the gram stain reaction of strep throat?
Streptococcus pyogenes is gram positive bacteria.
What is the different reaction of Gram-negative and Gram-positive bacteria to Gram's stain testing?
Gram-positive bacteria which take up the stain turn purple, while Gram-negative bacteria which do not take up the stain turn red.
Are acid fast bacteria gram positive or negative?
it is positive
What kind of bacteria can harm animals?
Just about any - gram negative, gram positive and acid-fast are all known to have pathogenic bacteria within them.
How does acid-fast bacteria differ from gram bacteria?
Acid-fast and Gram stain are two different types of staining technique. Bacteria that stain with acid-fast techniques are called "acid fast bacteria." Bacteria that don't stain with acid-fast techniques are called "not acid fast." At the same time, bacteria can be "Gram-positive" or "Gram-negative." For example, Nocardia is a bacterium that is acid-fast and Gram-positive. Usually the reason people care about these designations is in order to figure out what species a bacteria is. The issue usually arises in the context of a patient's sample that has grown bacteria. At first the doctors won't be sure which bacteria it is. They'll have guesses based on how the patient presented, but they won't know, and they want to know because that will help them pick the best treatment. A first step toward "speciating" the bacteria is to do a Gram stain on it. This is because for historical reasons determining whether a bacteria is Gram-positive or Gram-negative goes a long way toward speciating it. Acid-fast staining is less common, and is used mostly for diagnosing tuberculosis (caused by Mycobacteria, which are acid-fast bacteria). There are details about the cell walls that determine whether an organism will stain with Gram stain (thick walls without mycolic acids) or acid-fast, but hopefully this answers your question.
The major criteria used in placing bacteria into different groups is based on differences in?
Biologists separate bacteria into two groups based on their reaction with the gram stain as Gram Positive and Gram Negative bacteria.
What does a gram reaction of a cell refer to?
the Gram reaction is based on the structure of the bacterial cell wall. In Gram-positive bacteria, the dark purple crystal violet stain is retained by the thick layer of peptidoglycan which forms the outer layer of the cell. In Gram-negative bacteria, the thin peptidoglycan layer in the periplasm does not retain the dark stain, and the pink safranin counter stain stains the peptidoglycan layer. In other word,the gram reaction refers to how the cells reacts to the gram-staining process.
Is klebsiella gram positive or negative?
i believe it is a gram bacteria which causes respiratory illness.
Difference between the gram stain ans acid fast stain reactions of E. coli?
Gram staining is useful in separating bacteria into two groups: Gram positive or Gram negative. They are separated into these groups based on their cell wall structure. Gram positive bacteria contain a thick layer of peptidoglycan in their cell walls, while Gram negative bacteria contain a very small layer of peptidoglycan (15% or less of what Gram positive cell walls contain). A primary stain is added, such as Crystal Violet, that will stain all of the bacteria. Then, a mordant (such as iodine and potassium iodide) is added to form a complex between the peptidoglycan and the stain, which will make the cell more resistant to decolorization. Then, a decolorizing agent is added, which will remove the primary stain from Gram negative bacteria, but will cause the cell walls in Gram positive bacteria to dehydrate, and therefore, they will retain the primary stain. Finally, a counterstain (typically safranin) is added to distinguish Gram positive from Gram negative. Gram positive cells will be purple, and Gram negative cells will be red if Crystal Violet and Safranin are used.Acid-fast staining is entirely different. Is is used to detect species of bacteria in the genera Mycobacteria and Nocardia. These bacteria are resistant to typical staining procedures, such as Gram staining, due to a thick, waxy lipid layer in the cell wall composed of mycolic acid. Heating of the bacteria with a very strong stain such as carbol-fuchsin is necessary to "melt" this lipid layer, and force the stain through the cell wall. Once the bacteria has cooled, they will be incredibly resistant to decolorization. Non-acid fast bacteria do not contain this mycolic acid layer, and therefore, they will decolorize much easier, and are then stained with a counterstain to distinguish Acid-Fast bacteria from Non-Acid-Fast bacteria.
Is mycobacterium phlei gram positive or negative?
Although mycoplasma lack a cell wall and therefore test gram negative, they are considered to be descendents of 'nonsporulating and endospore forming gram-positive bacteria' (Madigan et al., 2009), such as Lactobacilli, Bacilli and Streptococci, (Dandekar et al., 2002), which have lost their cell wall. Mycoplasmas are therfore classed as gram- positive bacteria. This is supported by ribosomal RNA and DNA analysis.
What are examples of acid-fast organisms?
Two acid-fast bacteria that are pathogenic are:1. Mycobacterium tuberculosis2. Mycobacterium avium- Microbioman
