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H2S (hydrogen sulfide) Test:

This test is used to check for Enteric gram negative bacilli by checking for the enzyme thiosulfate reductase which is produced by various intestinal microorganisms. So if bacillus subtilis can produce this enzyme then this test will give you a pos. (black in color) result.

Yeah, no kidding. I'm pretty sure the question was what the result of it, or they wouldn't specify that it was b. subtilis. and it is positive for H2S production.

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Q: What is the hydrogen sulfide test used on Bacillus subtilis?
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