H2S (hydrogen sulfide) Test:
This test is used to check for Enteric gram negative bacilli by checking for the enzyme thiosulfate reductase which is produced by various intestinal microorganisms. So if bacillus subtilis can produce this enzyme then this test will give you a pos. (black in color) result.
Yeah, no kidding. I'm pretty sure the question was what the result of it, or they wouldn't specify that it was b. subtilis. and it is positive for H2S production.
It is NEGATIVE
No, it is negative.B. subtilis on EMB agar:http://www.microbelibrary.org/microbelibrary/files/ccImages/Articleimages/Atlas_EMB/Bacillus-subtilis_EMB_fig11.jpg
Hydrogen Sulfide production, Motility, and Indole production.
Bacillus cereus is oxidase negative -
Bacillus and Clostridium are both Gram positive, spore forming, bacilli (rods).Bacillus sp. are aerobic.Clostridium sp. are anaerobic.After performing a catalase activity test, Clostridium will be negative and Bacillus will be positive for catalase activity.
blue/positive
It is NEGATIVE
yes it have positive reaction for ONPG test
No, it is negative.B. subtilis on EMB agar:http://www.microbelibrary.org/microbelibrary/files/ccImages/Articleimages/Atlas_EMB/Bacillus-subtilis_EMB_fig11.jpg
Bacillus subtillus will test positive when performing a VP and Citrate test. Also, bacillus cereus is much more motile. It isn't as dependable as the chemical tests, but the cereus colonies will appear wispy and more widespread on an agar plate than those of subtilis.
Hydrogen sulfide is tested with lead acetate.
bacillus megatarium test positive for sucrose where creus doesnt
Hydrogen Sulfide production, Motility, and Indole production.
peptone agar is used to show the production of hydrogen sulfide
Place a piece of Lead acetate into the possible source. If Hydrogen Sulfide is present, the paper will turn black.
A measure of determining the effectiveness of a disinfectant using serial dilutions. definition found in intro to microbiology by Tortora, Funke, and Case.
negative!