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What is the insert capacity of a cosmid vector in DNA cloning?
Arvindkataria
The insert capacity of a cosmid vector is about 35-45 kb.
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∙ 13y ago
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What are phage vectors?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".Commonly used vectors include plasmid, Lambda phage, cosmid and yeast artificial chromosome (YAC).
What is the insert capacity of PACs vectors in DNA cloning?
In a M13 phage we can insert about 1-4 kb of target DNA sequence which is its maximum insert capacity.
Is a cloning vector a plasmid?
plasmid is the type of the cloning vector. other cloning vectors includes cosmids, bacteriophage, phagemids, artifiical chromosomes. clonong vectors are the carriers of certain traits to be inserted in non coding regions of the DNA.
How can you know donored-vector is cloning vector or expression vector?
Expression vectors contain translation start and termination codons. Moreover, it contains promoter region
The host organism into which a cloning vector is placed is called?
Competent cells if i not mistaken
What is the insert capacity of a lambda bacteriophage vector in DNA cloning?
We can insert about 5-25 kb sized foreign DNA in a lambda bacteriophage vector.
What is the cloning capacity of pBR322 vector?
The cloning capacity of pBR322 vector is 1-5kb.
What is the insert capacity of a P1 phage in DNA cloning?
You can add maximum 70-100 kb of genetic material in a P1 phage vector.
What is a small double stranded DNA that's used as a vector?
it is a ds DNA use in recombinant DNA technology to insert our interested gene and multiply it.Ex;plasmid,cosmid
What are phage vectors?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".Commonly used vectors include plasmid, Lambda phage, cosmid and yeast artificial chromosome (YAC).
Is a plasmid a cloning vector?
i have no idea wat plasmid,cloning ,and vector means so haha 2 u
How you clone a gene using cosmid as a vector?
The first step is restriction of the cosmid and the foreign DNA with the restriction enzyme, then ligating the fragments together. Thereafter, the cosmids are loaded into the phage capsid, which leads to the expression of the foreign gene through transduction.
What is the insert capacity of PACs vectors in DNA cloning?
In a M13 phage we can insert about 1-4 kb of target DNA sequence which is its maximum insert capacity.
Is a cloning vector a plasmid?
plasmid is the type of the cloning vector. other cloning vectors includes cosmids, bacteriophage, phagemids, artifiical chromosomes. clonong vectors are the carriers of certain traits to be inserted in non coding regions of the DNA.
Why is cloning an example of cloning?
Gene Cloning is used to clone a gene of interest in a vector called plasmid. The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. producing vector containing inulin gene is an example.
Is heat capacity a vector or a scalar?
Vector
By which of the strategies can you identify a foreign DNA into a vector?
You can identify the ligated DNA insert into a vector by doing DNA double digestion. Let say you inserted your foreign DNA with restriction sites Sma I and EcoRI. After ligation, you can digest the amplified chimeric rDNA with the same restriction enzyme. You can find the vector and the foreign insert on the resolved gel clearly if your cloning and digestion work properly.You can also confirm this by DNA sequencing.
