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Q: What is the major advantage of the gram stain over the simple stain?
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What is the advantage over the simple stain?

Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.


Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory Under what circumstances might the simple stain be preferable to the gram stain technique?

There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.


Why do all cells stain purple in the flagella stain but not in the gram stain?

The gram stain uses a decolorizing product so it is possible to differentiate between the gram and the gram cells. Gram positive cells stain purple in color.


Theories of principle of gram stain reactions?

Gram stain


Why it is necessary to prepare a bacterial smear?

For classification and identification purposes. Gram+ stain a dark purple/blue color while Gram - stain a red/pink color. This is due to the fact that Gram-positive cells have more peptidoglycan in their cell wall. Gram-negative cells don't have a think layer of peptidoglycan. They do have an outer layer of lipopolysaccharides and proteins which the Gram + does not have.

Related questions

What is the advantage over the simple stain?

Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.


What is the advantage of the gram stain over the simple stain?

gram staining differentiates between gram negative and gram positive bacteria by showing different colors. it shows blue or purple like color for gram positive bacteria and red color for gram negative bacteria. where as simple stain gives the same color to all the types of bacteria. hence it is difficult to differentiate between them.


Can you tell an organisms gram stain by simple staining?

Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).


Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory Under what circumstances might the simple stain be preferable to the gram stain technique?

There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.


What is the advantage of the gram stain over simple stains such as methylene blue?

Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.


What does a simple stain of bacillus megaterium look like under a microscope?

Simple or gram? Simple uses methylene blue, gram uses crystal violet


What is determined of the Gram Stain?

A gram stain is a cow


Why do all cells stain purple in the flagella stain but not in the gram stain?

The gram stain uses a decolorizing product so it is possible to differentiate between the gram and the gram cells. Gram positive cells stain purple in color.


Theories of principle of gram stain reactions?

Gram stain


What is the gram stain for cholera?

Gram Negative


Why it is necessary to prepare a bacterial smear?

For classification and identification purposes. Gram+ stain a dark purple/blue color while Gram - stain a red/pink color. This is due to the fact that Gram-positive cells have more peptidoglycan in their cell wall. Gram-negative cells don't have a think layer of peptidoglycan. They do have an outer layer of lipopolysaccharides and proteins which the Gram + does not have.


Can a Gram stain a prion?

Protists are often stained using a silver stain, not a Gram stain.