Vitrification and Morphological Variations - Some shoots developed in vitro appear brittle, glassy and water-soaked; this is called vitrification or hyperhydracity.
In many species, vitrification may be represented by symptoms not visible to the naked eye, e.g., poorly developed vascular bundles, abnormal wax quality, abnormal functioning stomata, etc. Vitrification is the consequence of culture conditions, and leads to losses of plantlets.
It may be overcome by the following:
(i) increased agar levels (Ca. 1 %),
(ii) bottom cooling of culture vessels,
(iii) addition of agar hydrolysates, and
(iv) use of growth retardants.
Morphological variants may arise during the multiplication state. Such variants occur, in most cases, at a frequency comparable to that in vivo. But in commercial ventures using meristem cultures, visual selection is practised to eliminate variants and maintain the homogeneity of plantlets produced.
Many commercial enterprises, therefore, prefer to multiply shoots for only four or so cycles from an explant; after this, a fresh batch of cultures is initiated from field-tested plants.
vitrification is also known as Hyperhydracity of the plant body
ex plant are nothing but the part of plant that can be used for plant tissue culture processes like for the regeneration of hybrid plants and so on .
The functions of a plant tissue culture labaroatory is to clone plants by taking an explant off an already existing plant in order to get another plant with exatly the same genetic make up.
Asexual Reproduction
Using Tissue culturemany plant can be grown from one parent in disease-free conditions.
Callogenesis is the shoot development in callus, due to low auxin and hign cytokinin concentration in the culturing medium during tissue culture.
In plant tissue culture, cells of plants are cultured. In tissue culture, cells (of plants, animals, bacteria, etc.) are cultured. Plant tissue culture is just like a subheading under tissue culture
by tissue culture method
tissue culture of mango se.
ex plant are nothing but the part of plant that can be used for plant tissue culture processes like for the regeneration of hybrid plants and so on .
Lii Jang Liu has written: 'Tropical plant cell and tissue culture' -- subject(s): Plant biotechnology, Plant cell culture, Plant micropropagation, Plant tissue culture
Roberta H. Smith has written: 'Plant tissue culture' -- subject(s): Laboratory manuals, Plant tissue culture 'In Vitro Propagation of Kalanchoe (Avery's Plant Tissue Culture Series)'
Plant tissue culture usually takes some time to grow. Depending on the culture taken, it can take a couple weeks.
using tissue culture many plant can be grown from one parent in disease free condition
plant tissue culture
S. S. Bhojwani has written: 'The embryology of angiosperms' 'Plant tissue culture' -- subject(s): Plant tissue culture, Bibliography
The functions of a plant tissue culture labaroatory is to clone plants by taking an explant off an already existing plant in order to get another plant with exatly the same genetic make up.
Cloning of crop plants in tissue culture involves tiny pieces from the parent plant. Sterile agar jelly with plant hormones are needed which makes tissue culture an expensive way of cloning crop plants.