We would need to know the path length and the molar extinction coefficient to answer that question. If you know these, it's an extremely simple matter of Beer's Law and algebra.
Because when we read absorbance, it's the amount of light absorbed by the bacteria itself. Absorbance is directly related to the amount of bacteria. More absorbance = more bacteria.
If you have a spectrofotometer ( the thing to mesure the absorbance) then play with the setting and use a maximum. this will lay close to your specific absorbance or take the pharmacopea or a MERCK index
because that chart gives a more accurate value than the absorbance scale on the specthometor
You need a graphic concentration versus absorbance.
to ensure maximum absorbance of light by the solution
"absorbance"Since in the experiment, you probably choose the wavelength, then measure the absorbance (absorption?, the absorbance is the dependent variable.
Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.
Blank samples are used to establish a baseline measurement of background contamination in an analysis. By analyzing a blank sample containing no target analytes, researchers can identify and account for any background signals or contamination that may affect the accuracy of their results.
specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com
in primary light absorbed by outer molecule while in secondary re-absorbance occurs
Because when we read absorbance, it's the amount of light absorbed by the bacteria itself. Absorbance is directly related to the amount of bacteria. More absorbance = more bacteria.
If you have a spectrofotometer ( the thing to mesure the absorbance) then play with the setting and use a maximum. this will lay close to your specific absorbance or take the pharmacopea or a MERCK index
A
because that chart gives a more accurate value than the absorbance scale on the specthometor
You need a graphic concentration versus absorbance.
to ensure maximum absorbance of light by the solution
Molarity is an indication for concentration.