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Most likely, shortly after the first humanoid figured out how to pick up a stick and use it as a weapon. That is, likely sometime well before civilization began, when humans were still hunter-gatherers. And, possibly well before humans (as a species) existed - there is very strong evidence to suggest that several ape species engage in warfare, which would almost certainly mean that pre-humans did too.

So, maybe as much as several million B.C.E.

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12y ago
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13y ago

Adam and Eve's battle over the apple

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Q: What is the oldest known battle?
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