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Phenols are acidic substances (pH over 7).
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∙ 6y ago
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What is bromophenol blue used for?
It is used as a pH (acid-base) indicator, particularly for substances with an expected pH between 3.0 and 4.6. Bromophenol blue turns yellow at or below a pH of 3.0. It turns purple at or above a pH of 4.6.
What are some of the most common acid and base indicators?
Phenolphthalein Methyl Orange Litmus Bromophenol Blue
What is the role of bromophenol blue is plasmid DNA isolation?
Bromophenol blue is the tracking dye in electrophoresis. Being of small molecular size, it races towards the other electrode before the DNA. It is used so that you don't mistakenly let the DNA get washed off the gel and into the buffer solution.
What is the blue band at the bottom of the gel when PAGE gel is performed and where are the DNA fragments?
The blue band at the bottom of the gel is Bromophenol blue and DNA fragments will be in the gel according to their molecular weight.
How do I prepare bromophenol indicator solution?
classic recipes say 0.25% bromphenol blue (0.25g/100ml) in a solution containing a viscous substance like: 40%sucrose, or 15%Ficoll, or 30%glycerol all in water. Personally, I use glycerol.
What is the colour of bromophenol blue when neutral?
blue
Why two stains required in SDS PAGE- bromophenol blue and coomassie blue?
bromophenol blue is used as the tracking dye while coomassie brilliant blue is the staining dye.
Why bromophenol blue is blue color?
At pH 3.0 it has blue colour
What does dye contain?
The loading dye comprises bromophenol blue, Ficoll 400 and water majorly while Xylene cyanol, Tris and EDTA are optional in it. Bromophenol blue is one of the most popular indicators of DNA in agarose gel electrophoresis. Bromophenol blue is a pH indicator.
Which is more polar bromophenol blue or phenolphthalein?
phenolphthalein
Can you use bromophenol blue in place of bromothymol blue?
no.heres why:bromophenol-blue-1bromothymol-blue-1
Can you use bromothymol blue in place of bromophenol blue?
no.heres why:bromophenol-blue-1bromothymol-blue-1
What is the Composition of gel loading dye used in DNA amplification?
Sucrose and Bromophenol blue (6X): 4gm sucrose 25mg bromophenol blue (0.25%) Distilled water to 10 ml
Does Amax of bromophenol blue vary with concentration?
Bromophenol Amax or maximum absorbance in spectrophotometer is 590nm I guess so hehe^^ based on my experimental result though!
What is bromophenol blue used for?
It is used as a pH (acid-base) indicator, particularly for substances with an expected pH between 3.0 and 4.6. Bromophenol blue turns yellow at or below a pH of 3.0. It turns purple at or above a pH of 4.6.
What color is bromophenol blue?
bromothymol blue is an indicator for carbon fioxide, so if it touches carbon dioxide, it turns yellow.but by itself, it is blue.
If you add a bromophenol blue solution to .01M HCl what solution color would result after mixing?
yellowish
