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By this technique, we can diffentiate the acid fast and non acid- fast bacteria. The non acid-fast bacteria are M.tuberculosis and N.asteriodes. They are the causative agents for tuberculosis and nocardiosis respectively. The acid fast staining or the Ziel- Nielsen's staining is the only procedure to find out the above mentioned pathogens.

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Q: What is the practical importance in a medical lab of the acid-fast stain?
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