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To determine the order of nucleotides in a DNA molecule
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Why are ddNTPs used for DNA sequencing?
Because on the addition of the normal ddNTP to the sequencing, there will be addition of another nucleotide added by DNA polymerase which keeps DNA sequencing going.
What is the purpose of the modified bases in DNA sequencing?
- DNA replication terminates when a modified base is added. - They are easily recognized by the computer. - They are added to the DNA chain just like the normal bases.
What are some methods of sequencing DNA?
Some methods that are sequencing DNA is utilizing labeled nucleotides for corporation into a copy of a piece of DNA. The DNA segment to be copied, called the template DNA, is separated into two strands by heating.
What is error rate in DNA sequencing?
n
How do you match a DNA sequence?
DNA sequence can be match by DNA sequencing method and it is of following type given by different scientist:1 The Sanger-Coulson method - this is chain termination method2 The Maxam-Gilbert method - this is chemical degratation of DNA3 By sequencing PCR products4 Automated DNA sequencing
Why are ddNTPs used for DNA sequencing?
Because on the addition of the normal ddNTP to the sequencing, there will be addition of another nucleotide added by DNA polymerase which keeps DNA sequencing going.
Who discovered the technique of DNA sequencing?
DNA sequencing was first discovered by Fredrick sanger in 1950s
What is the main purpose of doing dna sequencing?
DNA sequencing is a laboratory technique used to determine the exact sequence of bases (A, C, G, and T) in a DNA molecule. The DNA base sequence carries the information a cell needs to assemble protein and RNA molecules. DNA sequence information is important to scientists investigating the functions of genes.
Describe what is meant by the term rapid sequencing?
Sequencing DNA rapidly
What is the purpose of the modified bases in DNA sequencing?
- DNA replication terminates when a modified base is added. - They are easily recognized by the computer. - They are added to the DNA chain just like the normal bases.
Dideoxyribonucleotide chain-termination is a method of?
Sequencing DNA (Sanger Sequencing Method).
What does not read DNA sequences?
People not versed in DNA sequencing.
What is the concept of genetic sequencing about?
When looking for information about the sequence of DNA then there is information relating to the concept of genetic sequencing available from Wikipedia. The site offers about DNA sequencing with links that relate to other facts and information on the different aspects of genetic sequencing.
What is meant by pyrosequencing?
It is common knowledge that pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle.
How is DNA tested and why?
by DNA fingerprinting method , DNA-DNA hybirdization or DNA sequencing. to know the sequence of DNA
Determining the exact order of nucleotides in DNA is called?
DNA Sequencing
What does guanine combines with?
In DNA sequencing, Adenine and Guanine are known as "base pairs", and are purines, which form the building blocks of DNA and RNA. Guanine combines with Adenine in DNA sequencing.
