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Why chilled ethanol is added for DNA isolation?
to precipitate extracted DNA
Can you use spirit for DNA precipitation?
Several DNA isolation protocols recommend the use of either ethyl or isoamyl alcohol for the precipitation step
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
Where do you get the DNA fragments for Gel electrophoresis?
The DNA fragments comes from the method of DNA isolation.
Why chilled ethanol is added for DNA isolation?
to precipitate extracted DNA
Why 70 percent alcohol and absolute alcohol used in DNA isolation?
70% ethnol is used for the dna isolation becuse it makes hydrogen bonding with the water molecules and makes DNA hydrophopic so that it pricipitated.
Can you use spirit for DNA precipitation?
Several DNA isolation protocols recommend the use of either ethyl or isoamyl alcohol for the precipitation step
What is the purpose of alcohol when extracting DNA?
According to me, we use alcohol because DNA is insoluble in alcohol, it aggregates together, giving a pellet in centrifugal and we can see a precipitated DNA with naked eyes (that we suppose to see in experiment i.e DNA extraction)....
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
What is the principle involved in the isolation procedure?
DNA isolation is a based on the principle of purification. DNA samples are isolated through the use of physical and chemical methods. Friedrich Miescher conducted the first isolation of DNA in 1869.
Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
What is role of Cetyl trimethyl ammonium bromide in DNA isolation?
it act as as a cationic detergent for the isolation dna from the given sample
Role of phenol chloroform in DNA isolation?
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
Where do you get the DNA fragments for Gel electrophoresis?
The DNA fragments comes from the method of DNA isolation.
What are the function of sodium acetate in dna isolation?
Sodium acetate buffer helps by reacting with the membrane protein and precipitating them, thus facilitating the dna isolation.
What materials do scientists typically use when extracting DNA?
phenol,chloroform,isoamyl alcohol,ethanol,CTAB reagent,Na acetate etc.. but nowadays, they use kits for any kind of DNA isolation, which makes their job easier.
