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What is the purpose of inoculating tsa plates with samples from the zone of inhibition?
Wiki User
∙ 13y ago
Any bacteria that grow in the zone of inhibition are resistant to the antibacterial used. By inoculating new plates with the bacteria, you will have a pure culture of resistant bacteria.
Wiki User
∙ 13y ago
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Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
Why do you use an inoculating loop instead of a needle to make the transfer from the culture plates to the culture tubes?
Because the solid media is more dense over a smaller area so a inoculating needle is used to retrieve the specimen. Where as for a liquid medium the specimen is more spread out over the liquid. The inoculating loop can collect more liquid because there is more metal present at the inoculating specimen retrieval point and has the ability to collect liquid in the loop. I'm currently taking general microbiology and my lab book hardly covers this. A.C.
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