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Answer by NO.1GreatThinker :-
The basic purpose of the negative staining experiment is to check out the bacteria / micro-organisms which have less amount of peptidoglycans present in their cell wall
So experiment can be performed to distinguish gram negative bacteria from gram positive one .
It is performed by using a dye nigrosin which is a negatively charged dye in nature .
So it is first taken on a slide ( just a drop of it ) .
Then specimen of the bacteria is taken from the culture .
It is placed over the dye .
Now since bacteria is also negatively charged . So there is repulsion in both dye and the bacteria . Hence the bacteria seems or appears in between the dye as bright light spots . It is observed under the light microscope .
Hence the experiment .
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∙ 12y ago
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What is the best use of negative staining?
size and shape
Why can treponema denticola be seen with negative staining?
Because it is a Gram-negative bacterium and can be stained with Gram-negative stain.
What is the definition of negative staining?
negative staining is when the micro-organism on slide is not stained.. instead the rest of the material on the slide gets stained and the organism stands out prominently unstained.. this occurs because that organism has a thick capsule around it which is non-stainable with that stain.. example is pneumococci staining with India ink.
What is thee purpose of adding methylene blue to the specimen?
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
Why can tooth and gum bacterium be seen with negative staining?
Not all oral bacteria are gram negative, but those that are there can be seen with Gram stain.
What is the different staining technique in virology?
1- What_is_the_different_staining_technique_in_virology2- What are the diffrent stain in micro for virus ?
What is the purpose of spore staining?
not sure
What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
What is the purpose of staining?
to detect bacterial stracture
What is the other name for Negative staining?
Capsule Stain
What is the best use of negative staining?
size and shape
What is Gram staining method used for?
the purpose is to determine diffrent kinds of bacteria based on their results. if it'sgram positive the color will be pink and if it is gram negative the color will be red.
What is the purpose of albert staining?
to detect bacterial stracture
Why can treponema denticola be seen with negative staining?
Because it is a Gram-negative bacterium and can be stained with Gram-negative stain.
What types of organisms are subjected to Negative Staining Technique?
Bacteria .
The factor in an experiment that is changed on purpose?
the factor an experiment that is changed on purpose
Why is the technique called negative staining?
Because the cell wall repels the binding of the negative stain therefore the cells do not stain. Because of this the background is stain with the dye used and the bacteria remain colorless. Basically your staining the background, that is, you are not directly staining the cells.
