To determine the order of nucleotides in a DNA molecule
I myself is asking for answer
Because on the addition of the normal ddNTP to the sequencing, there will be addition of another nucleotide added by DNA polymerase which keeps DNA sequencing going.
- DNA replication terminates when a modified base is added. - They are easily recognized by the computer. - They are added to the DNA chain just like the normal bases.
Some methods that are sequencing DNA is utilizing labeled nucleotides for corporation into a copy of a piece of DNA. The DNA segment to be copied, called the template DNA, is separated into two strands by heating.
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DNA sequence can be match by DNA sequencing method and it is of following type given by different scientist:1 The Sanger-Coulson method - this is chain termination method2 The Maxam-Gilbert method - this is chemical degratation of DNA3 By sequencing PCR products4 Automated DNA sequencing
Because on the addition of the normal ddNTP to the sequencing, there will be addition of another nucleotide added by DNA polymerase which keeps DNA sequencing going.
DNA sequencing was first discovered by Fredrick sanger in 1950s
DNA sequencing is a laboratory technique used to determine the exact sequence of bases (A, C, G, and T) in a DNA molecule. The DNA base sequence carries the information a cell needs to assemble protein and RNA molecules. DNA sequence information is important to scientists investigating the functions of genes.
Sequencing DNA rapidly
- DNA replication terminates when a modified base is added. - They are easily recognized by the computer. - They are added to the DNA chain just like the normal bases.
Sequencing DNA (Sanger Sequencing Method).
People not versed in DNA sequencing.
When looking for information about the sequence of DNA then there is information relating to the concept of genetic sequencing available from Wikipedia. The site offers about DNA sequencing with links that relate to other facts and information on the different aspects of genetic sequencing.
It is common knowledge that pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle.
by DNA fingerprinting method , DNA-DNA hybirdization or DNA sequencing. to know the sequence of DNA
In DNA sequencing, Adenine and Guanine are known as "base pairs", and are purines, which form the building blocks of DNA and RNA. Guanine combines with Adenine in DNA sequencing.
DNA Sequencing