0
What is the role of Cloning Vector in recombinant DNA technology?
Wiki User
∙ 12y ago
The desired gene is cut from its original DNA by using restriction enzyme. The vector (plasmid) is also cut with the same restriction enzyme so that both the vector and the desired gene have the same sticky end. They are bonded together by DNA ligase.
Why desired gene are cloned into vector? This is because vector have the ability to reproduce by itself and at the same time carry the desired gene together; in another word, it can replicated the desired gene in the host cell.
Wiki User
∙ 11y ago
Wiki User
∙ 12y agoThe cloning vector, or host, will anneal with your desired DNA fragment and express this gene as it replicates.
Add your answer:
Earn +20 pts
What is the role of Cloning Host in recombinant DNA technology?
The role of cloning host in RDT is to serve as the host cell under culture, in which the designed gene is subjected to produce recombinant protein.
Explain the role of klenow fragment in recombinant DNA technology?
single strand to double strand
What is the role of science and technology in communication and transportation?
Science and technology are absolutely vital to all of those fields.
What is the role of cosmids in recombinent DNA technology?
Cosmids allow you to insert DNA sequences (genes) into the genome of a bacteria, much like a plasmid. Cosmids in recombinant DNA technology, however, utilize phage (bacterial viruses) to enable researchers to insert DNA sequences of genes with up to 44,000 base pairs, while normal plasmids are only able to efficiently carry 10,000 base pairs. This allows researchers to insert more complex genes into bacteria as well as increases the efficiency with which researchers can make genomic libraries of more complex organisms.
The role of restriction enzymes in DNA technology is to?
Produce a cut (usually staggered) at a specific recognition sequences on DNA.
What is the role of Cloning Host in recombinant DNA technology?
The role of cloning host in RDT is to serve as the host cell under culture, in which the designed gene is subjected to produce recombinant protein.
Explain the role cloning vectors play in making recombinant DNA?
they carry a gene like a vehicle
Explain the role of klenow fragment in recombinant DNA technology?
single strand to double strand
What role does E. coli play in recombinant DNA Technology?
It helps to create human insulin.
What is the role of x-gal iptg in selection of recombinants?
to differenciate recombinant dna from non recombinant
What is Role of spreading vector disease?
what is the role of a vector in the spread of a disease
What role does imprinting play in cloning humans?
no
What role should the government play on cloning?
meow
What is the role of a vector?
To indicate the direction.
What is gene cloning and why do you need to clone a gene?
Gene cloning is the technique of recombinant DNA technology in which a desired gene of interest having a striking characteristic feature is cloned. The gene may be selected because it appears to influence the organism in a striking manner, or to determine the role of the gene in the organism. Genes can be clones for industrial purposes, for instance the production of vaccines and insulin, or for research purposes, to determine what the role of the gene is. Gene cloning requires a basic knowledge of the gene's sequence, or flanking sequences. Genes can be cloned using polymerase chain reaction (PCR), if the sequence is known, or by cutting genomic DNA with restriction enzymes (to create smaller chunks of DNA). Usually, once a fragment containing gene has been identified using restriction enzymes, it is sequenced and PCR is used to isolate the specific sequence within the fragment.
What is the role of the enzymes in cloning?
The role of arestriction enzyme is to remove the intronsto make a mature mRNA, so that the new mRNA codes for the making of a single protein. The restriction enzyme will only leave the exons to make the mRNA more shorter, and therefore easier to insert into plasmids
What is the role of technology in education?
The role of technology in learning is to teach children how the skills of computers and technology so when they get a job if it involves computer technology they are able to complete the tasks.
