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What is the role of EtBr in electrophoresis?
Ethidium bromide interchalates with DNA. It doesn't affect electrophoresis, but it help visualise the DNA bands after electrophoresis. The EtBr that is bound to the DNA will fluoresce under ultraviolet light.
What does the company Stratagene manufacture?
Stratagene is a biotechnological company established in 1984. Its fields include cellular analysis, cloning, cytogenomics, DNA methylation and DNA sizing and quantification, and food testing.
What are the methods of preparing DNA for forensic analysis?
DNA for forensic analysis is typically prepared using methods such as DNA extraction from biological samples, quantification of DNA concentration, amplification of specific DNA regions using PCR, and analysis of the DNA profiles through techniques like gel electrophoresis or DNA sequencing. Additionally, DNA samples are often treated with chemicals to remove contaminants and prevent degradation before analysis.
How the structure of DNA aids in each role?
DNA has a role in defining characteristics of the subject which it is taken from.
What role does deoxyribose play in DNA?
It is the sugar in DNA
What is the role of integrase in HIV?
The role integrase plays in HIV is that it fuses viral DNA with host DNA.
What is the role of DNA ploymerase in DNA replication?
Happy poo
What is a Taqman Real Time PCR?
Taqman Real Time PCR is a technique used in molecular biology to quantify the amount of a specific DNA target present in a sample. It involves the use of specific probes that bind to the target DNA sequence and produce a fluorescent signal during amplification, allowing for real-time detection and quantification of the DNA target. This method is widely used in research, clinical diagnostics, and other applications requiring accurate quantification of DNA.
What is the function of the fluorescent DNA stain?
Fluorescent DNA stains are used to bind specifically to DNA, allowing for visualization and quantification of DNA in various samples. They emit fluorescence when excited by specific wavelengths of light, making it possible to detect and analyze DNA in techniques such as gel electrophoresis, microscopy, and flow cytometry. These stains are crucial for applications in molecular biology, including studying gene expression, DNA fragmentation, and cell cycle analysis.
What are the key components and steps involved in using a cell-free DNA extraction kit for isolating cell-free DNA from biological samples?
The key components and steps involved in using a cell-free DNA extraction kit for isolating cell-free DNA from biological samples include: Sample collection and preparation Lysis of cells to release DNA Binding of DNA to a membrane or beads Washing to remove impurities Elution of purified DNA Quantification and analysis of extracted DNA.
What is the role of the DNA new strand?
It is a copy of the Dna original strand.
What is role of the DNA ligase in DNA replication?
It joins Okazaki fragments
