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Multiply the magnification or power of the objective lens times the power of the eyepiece and it equals the total magnification
On a microscope with the usual 3-lens turret it is usual to use the objective lens with the lowest magnification to first examine your specimen. This gives a wider overall view of the subject, and will allow you to choose the particular detail that best suits your study. You may then move on to a higher magnification, if necessary, to study finer detail. If you started with the highest magnification, your fine focus will be uncertain, and you risk the front of the objective lens coming into contact with the sample slide. This could damage your specimen, and may damage the front of the lens.
it could be either coarse or fine knob. coarse knob is used to focus image at higher magnification, while fine knob is used to focus on lower magnification
For visual observation: Magnification = (Focal Length of Objective Lens) divided by (Focal Length of Eyepiece) (They have to be measured in the same units.) For prime-focus photography: (One focal-length of the Objective Lens on the film) = (one radian in the sky)
We don't think you can do it with that information. 'f-stop' = (focal length of the objective lens) divided by (its diameter) Magnification of the scope = (focal length of the objective) divided by (focal length of the eyepiece) Looks like in order to calculate the 'f-stop', you need to estimate or measure the focal length of either the objective or the eyepiece. Here's an idea: If you can temporarily separate the objective from the tube, use the objective to focus an image of the sun on the sidewalk. (Not on anything flammable.) Measure the distance from the lens to the sharpest image. With the 'object' at infinity, the image is at the focal length.
parfocal
Multiply the magnification or power of the objective lens times the power of the eyepiece and it equals the total magnification
If magnification increases ONLY, then resolving power does not increase. However, if the magnification increased while staying in focus (upgrading resolution and magnification with objective lense), shorter wavelengths are needed to stay in focus with increased magnification to yield the same high resolution as with previous objective lense, so this case, resolving power does increase.
As magnification increases, the depth of focus decreases.
true
I assume this is a question about viewing an object through a compound microscope. Always use the shortest objective lens (that is the one with the lowest magnification) first. Focus the lens and then when you want more detail or a closer view, you can simply rotate the lens turret to a longer lens and it should be nearly in focus. Use the fine focus feature to sharpen the picture. If an object is viewed in this way, the danger of scratching the high power objective by ramming it into the slide is lessened.
what objective use to focus a microscope
On a microscope with the usual 3-lens turret it is usual to use the objective lens with the lowest magnification to first examine your specimen. This gives a wider overall view of the subject, and will allow you to choose the particular detail that best suits your study. You may then move on to a higher magnification, if necessary, to study finer detail. If you started with the highest magnification, your fine focus will be uncertain, and you risk the front of the objective lens coming into contact with the sample slide. This could damage your specimen, and may damage the front of the lens.
To bring a sharp focus.
The low power objective lens helps to first fine the specimen or object being viewed, and to focus the microscope. Once the lens power becomes higher it is much harder to focus the microscope and find the desired object of viewing.
it could be either coarse or fine knob. coarse knob is used to focus image at higher magnification, while fine knob is used to focus on lower magnification
All magnification