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What is the use of cDNA probes?

Updated: 9/25/2023
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Q: What is the use of cDNA probes?
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What is extracted from cells for DNA microarray technology?

mRNA is extracted from cells for DNA microarray. the mRNA is then converted in the lab to cDNA this cDNA is allowed to interact with the probes on the microarray chip


What is genomic chips?

Genome chips are miniaturized plates containing hundreds of microscopic wells on their surface. These wells contain DNA probes. DNA probes are basically stretches of cDNA from a particular genome. When genomic DNA isolated from an organism is allowed to interact with the cDNA probes, come probes bind to the genomic DNA while others do not (depending on complementarity. A laser light is used to read each well and look at what sequences are bound. This information is valuable to scientists who can determine changes in gene expression based on the information obtained from a gene chip


What is virtual cDNA library?

I imagine its just an online cDNA library. A cDNA library is of course a collection of cDNA copy sequences. cDNA is where you have mRNA and you use reverse transcriptase to turn a strand of RNA into a DNA equivalent, then use RNAase H to degrade the remaining RNA strand and then use DNA polymerase to create a complete double stranded DNA sequence that is the equivalent of the mRNA. This way you can get the gene without the introns that normal DNA would have.


What is the use of DNA library?

cDNA is the short form complementary DNA. cDNA libraries are a combination of cloned cDNA fragments. cDNA libraries are used to express eukaryotic genes in prokaryotes.


What is the full form of cdna?

CDNA = Complimentary Deoxyribose Nucleic Acid


How is cdNA created?

cDNA or complimentary DNA is created by a catalyzed reaction from DNA polymerase and reverse transcriptase which results in the use of mRNA or messenger RNA as template for DNA synthesis.


The function of cdna?

hi In vitro we must converted the RNA to cDNA to diagnosis viral RNA in PCR. In vivo RNa viral infected the cell RNA converted to cDNA IN SIDE THE CELL BY REVERSE TRANSCRIPTASE therfore cDNA insertion in the DNA of cell infected thank you hi In vitro we must converted the RNA to cDNA to diagnosis viral RNA in PCR. In vivo RNa viral infected the cell RNA converted to cDNA IN SIDE THE CELL BY REVERSE TRANSCRIPTASE therfore cDNA insertion in the DNA of cell infected thank you


CDNA is copied from?

mRNA


What is a cDNA library used for?

A cDNA library is used for complementary DNA. These DNA are collected as host cells, which can be found in the nucleus. Currently, cDNA libraries are lacking in the enhancer, intron, and several other categories.


What is the advantage of using cDNA library in molecular genetics?

The main advantage of cDNA library is that it contains only the coding region of a genome.


Does a cDNA library have only exons?

A cDNA library consists only of genes that are expressed, hence they do contain only exons. They contain no introns.


What is the purpose of the cDNA synthesis?

The purpose of cDNA synthesis is to synthesize a copy of DNA from mRNA. This means that it is involved in the duplication of DNA that occurs when a cell divides. As a result, without cDNA synthesis, life would not exist as cells would not be able to divide.