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What is the use of potassium phosphate in DNA extraction method?
Wiki User
∙ 11y ago
It servers as buffering agent to maintain a stable ph during the extraction/purififaction protocol; DNA is known to be most stable in neutral or slightly basic (pH7-8) solutions.
Furthermore, the phosphate may bind to surfaces that would otherwise bind the DNA(phosphate backbone of DNA!) thus keeping the latter in solution; this helps with samples containing low amounts of DNA.
Wiki User
∙ 11y ago
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If you fixed a tissue in ethanol that have methanol or other toxic material and then you extract DNA from it does the DNA functionable for PCR?
It depends on the method of extraction. Extraction that involve the use of resins of ionic exchange or affinity should be able to eliminate other substances.
What is the purpose of Trichloroacetic acid in DNA extraction?
Trichloroacetic acid is used for precipitation of the DNA during its extraction.
Where are phosphate groups in a DNA molecule?
The DNA backbone, are made of alternating sugars and phosphate groups.
What is the sequence of subunits in the DNA backbone?
The sequence of subunits in the DNA backbone is phosphate, sugar, phosphate, sugar, phosphate, and sugar. The coding region is the code for protein sequence.
Why grind the liver in a DNA extraction lab?
the purpose of grinding any substance during dna extraction is cell loosening.
Are there alternatives of potassium acetate in DNA extraction?
to take out the detergents
DNA extraction from bacteria?
DNA extraction from bacteria can be achieved in various ways. Yeast is the best resource to extract the DNA bacteria from using extreme rapid extraction method.
Why you add sodium acetate in dna extraction?
you add sodium acetate to neutralize the charge on the sugar-phosphate backbone of DNA
What is the uses and importance's of phenol chloroform method in DNA extraction?
importance of phenol
Will calcium chloride and potassium phosphate precipitate?
yes it will precipitate DNA if your lysing nuclei; add benzamidine hydrochloride though as a protease inhibitor.
If you fixed a tissue in ethanol that have methanol or other toxic material and then you extract DNA from it does the DNA functionable for PCR?
It depends on the method of extraction. Extraction that involve the use of resins of ionic exchange or affinity should be able to eliminate other substances.
Role of sodium chloride in DNA extraction?
Sodium chloride was needed to ensure the proteins in the cell aren't separated from the rest of the solution with the DNA.
Does DNA contain potassium?
No, DNA does not contain potassium.
What does sodium citrate do in DNA extraction?
how to make sodium citrate in 10% ethanol for DNA extraction
What is the function of CL buffer in DNA extraction?
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
What is the purpose of Trichloroacetic acid in DNA extraction?
Trichloroacetic acid is used for precipitation of the DNA during its extraction.
Are there any jobs that Involve DNA extraction?
DNA extraction is an invaluable technique in a majority of molecular biologytechniques. One must not restrict themselves to this method alone. Onceyou are familiarwithDNA extraction, learn how to work with plasmid, RNA and cDNA.Isolating and handling these nucleotides are an important skill in carrying out experiments in the fields of molecular biology and genetics
