methelyn blue
In the presence of heat and acetic acid, ortho-toluidine (also called o-toluidine) reacts rapidly with aldohexoses (mostly glucose) to form a green-colored complex that can be measured at 630 nm.
it is used to keep flys off monkeys butts
Methylene blue is used for many different staining purposes, but one of the main ones is staining RNA or DNA. In animal cells, it will stain the cytoplasm and the nucleus (the nucleus will be much darker).
Methylene blue
As expected from Inductive(/hypercojugative) as well as ortho effect the basicity order should be: ortho-toluidine>para-toluidine>meta-toluidine. But, experimental order is: para-toluidine(Pka=5.12)>meta-toluidine(Pka=4.69)>ortho-toluidine(Pka=4.39).The order of basicity is explained by two opposing effects :i) inductive effect ii) Stability of the conjugate base. Here 2nd factor is more significant for ortho-toluidine & 1st factor is important for para-toluidine. For ortho-toluidine the conjugate base [o-MeC6H4NH3+] is destabilized by steric interaction between -NH3+ & -Me(at ortho). This desatabilization is absent with para-toluidine & meta-toluidine.Tha's why basicity of: ortho-toluidine(Pka=4.39)
Stain with basic dyes cytoplasm shows blue precipitates
methelyn blue
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In the presence of heat and acetic acid, ortho-toluidine (also called o-toluidine) reacts rapidly with aldohexoses (mostly glucose) to form a green-colored complex that can be measured at 630 nm.
Methylene blue stain is used to stain plant and animal cells.
A
Yes, because the methylene blue stain makes the organelles in eucharyotic cells visible to us in a basic microscope.
There are several biological stains that can be used for plants. Some of the usual stains used on plants are toluidine blue, iodine, and Eosin Y.
Yes.
Para toluidine appears as white lustrous plates or leaflets with an amine odour at room temperature. It melts at 430C.
You can observe cheek cells