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What is toluidine blue stain?

Updated: 11/22/2022
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Q: What is toluidine blue stain?
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Compare the basicity of isomers of toluidine?

As expected from Inductive(/hypercojugative) as well as ortho effect the basicity order should be: ortho-toluidine>para-toluidine>meta-toluidine. But, experimental order is: para-toluidine(Pka=5.12)>meta-toluidine(Pka=4.69)>ortho-toluidine(Pka=4.39).The order of basicity is explained by two opposing effects :i) inductive effect ii) Stability of the conjugate base. Here 2nd factor is more significant for ortho-toluidine & 1st factor is important for para-toluidine. For ortho-toluidine the conjugate base [o-MeC6H4NH3+] is destabilized by steric interaction between -NH3+ & -Me(at ortho). This desatabilization is absent with para-toluidine & meta-toluidine.Tha's why basicity of: ortho-toluidine(Pka=4.39)


Does a reticulocyte stain with cresyl blue?

Stain with basic dyes cytoplasm shows blue precipitates


What stain is used to stain cheek cells?

methelyn blue


Why o-toluidine method cannot be used for the detection of fructose in a solution?

??


Why you use o-toluidine for glucose estimation?

In the presence of heat and acetic acid, ortho-toluidine (also called o-toluidine) reacts rapidly with aldohexoses (mostly glucose) to form a green-colored complex that can be measured at 630 nm.


What kind of stain are used to stain plant and animal cells?

Methylene blue stain is used to stain plant and animal cells.


What is the sensitivity and specificity of ortho toluidine test?

A


Is it useful to stain the Elodea and Anabaena cells with methylene blue stain?

Yes, because the methylene blue stain makes the organelles in eucharyotic cells visible to us in a basic microscope.


Biological stains used for plants?

There are several biological stains that can be used for plants. Some of the usual stains used on plants are toluidine blue, iodine, and Eosin Y.


Can methylene blue stain skin?

Yes.


Is p-toluidine a solid or a liquid at room temperature?

Para toluidine appears as white lustrous plates or leaflets with an amine odour at room temperature. It melts at 430C.


What can you observe when you stain a cheek cell with methylene blue stain?

You can observe cheek cells