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EcoR1 is a restriction enzyme (endonuclease), which splits the phosphodiester bonds of the backbone of DNA.
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∙ 14y ago
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How many times EcoR1 cuts the DNA to generate 4 fragment?
3 times
What kind of molecule is an enzyme-?
The type of molecule that is an enzyme is a protein molecule.
What kind of molecule is an enzynme?
A protein.
What kind of molecule has sugar and phosphate?
A nucleotide
Would it be easier for DNA ligase to reconnect two fragments cut by EcoR1 or one fragment cut by EcoR1 with one cut by Hind111?
Blunt or sticky ends are not a problem for the ligase, the thing is, the enzyme used should be the same to cut the insert and the vecotr(which may be a single or double enzyme but same!)
What kind of molecule is a polymer?
A polymer molecule is a macromolecule.
How many times EcoR1 cuts the DNA to generate 4 fragment?
3 times
What would happen if you cut both the jellyfish glo gene and puc18 plasmid with the ecor1 restriction enzyme?
If there is a EcoR1 site in either the middle of the Glo gene, or in the middle of the selectable marker site in the plasmid, it would likely disable either Glo, or the plasmid.
What kind of molecule is an enzyme-?
The type of molecule that is an enzyme is a protein molecule.
What kind of molecule is formed when many disaccharide molecule's are combined?
cellulose
What kind of molecule does translation produce?
The molecule produced after translation of RNA is protein.
What kind of molecule is an enzynme?
A protein.
Which kind of molecule is water?
inorganic
What kind of atoms are in a molecule of hydrogen?
A hydrogen molecule consists of two hydrogen atoms.
What kind off molecule has a positive end and a negative end?
A polar molecule such as water
What kind of molecule has sugar and phosphate?
A nucleotide
Would it be easier for DNA ligase to reconnect two fragments cut by EcoR1 or one fragment cut by EcoR1 with one cut by Hind111?
Blunt or sticky ends are not a problem for the ligase, the thing is, the enzyme used should be the same to cut the insert and the vecotr(which may be a single or double enzyme but same!)
