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Would it be easier for DNA ligase to reconnect two fragments cut by EcoR1 or one fragment cut by EcoR1 with one cut by Hind111?
Wiki User
∙ 11y ago
Blunt or sticky ends are not a problem for the ligase, the thing is, the enzyme used should be the same to cut the insert and the vecotr(which may be a single or double enzyme but same!)
Wiki User
∙ 11y ago
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What causes the bands on your DNA gels?
During gel electrophoresis, the DNA moves along the agarose gel to the positive side of the box, and after a certain amount of time, the smaller DNA fragments travel the farthest (because they have an easier time navigating the pores of the gel) and so on, leaving behind a series of bands comprised of similar-sized DNA fragments.
Do the smallest fragments in a gel electrophoresis end up on top or bottom?
Depending on where the died fragments start, the smallest parts end up way on the other side. The gel acts as a filter and the electrical current acts as... the current to push the fragments through the gel. Being that they're small... those fragments have an easier time getting through the gel. The bigger fragments are closer to where the fragments started cause they're big and have a harder time going through the gel. Eventually you should have like areas in the gel that look cool and CSI like, as if you were testing for DNA samples. Sadly that may not always happen, being as... well this is reality and not show biz. Good luck though on your next/first attempt.
What are the objectives of agarose?
The agarose gel acts as a matrix that slows down the dna segments as they move to the opposite charged end of the gel. A larger segment will have a tougher time moving through the gel, while a smaller segment will move faster because it is easier to move it through the gel.
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What causes the bands on your DNA gels?
During gel electrophoresis, the DNA moves along the agarose gel to the positive side of the box, and after a certain amount of time, the smaller DNA fragments travel the farthest (because they have an easier time navigating the pores of the gel) and so on, leaving behind a series of bands comprised of similar-sized DNA fragments.
Do the smallest fragments in a gel electrophoresis end up on top or bottom?
Depending on where the died fragments start, the smallest parts end up way on the other side. The gel acts as a filter and the electrical current acts as... the current to push the fragments through the gel. Being that they're small... those fragments have an easier time getting through the gel. The bigger fragments are closer to where the fragments started cause they're big and have a harder time going through the gel. Eventually you should have like areas in the gel that look cool and CSI like, as if you were testing for DNA samples. Sadly that may not always happen, being as... well this is reality and not show biz. Good luck though on your next/first attempt.
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