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It has a peptidoglycan layer filled with mycolic acids. The acids make the cell wall waxy and impenetrable to stains. They are classified with gram positive cells because of cell wall thickness and genetic similarities.
Wiki User
∙ 9y ago
Wiki User
∙ 16y agoBecause bacteria in this genus contain mycolic acid
Wiki User
∙ 12y agoBecause of its thick cell wall with the mycolic acids, mycobacterium does not respond very well to staining agents.
Wiki User
∙ 10y agothe presence of mycolic acid
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What relationship did you find if any between the Gram-staining reaction of a microorganism and its susceptibility to antiseptics and disinfectants?
Gram + bacteria has thick cell walls. This feature makes them more resistant to antiseptic and disinfectants.
What are the consequences of Ziehl-Neelsen staining?
The Ziehl-Neelsen stain is also known as the acid-fast stain. It contains sulfuric acid, and is used to identify acid-fast bacteria, or bacteria resistant to decolorization by acids from staining.
What is the scientific name of the leprosy bacteria?
Mycobacterium leprae and Mycobacterium lepromatosis
Rules in gram staining?
All cocci are gram (+) except Neisseria, Branhamella, Moraxella and Veilonella. All bacilli are gram (-) except Mycobacterium, Corynebacterium, Lactobacillus, Listeria, Clostridium, Bacillus, Erysipelothrix, and Nocardia. All spirals are gram (-)
What kind of stain do you stain Mycobacterium with?
For Mycobacterium you will use the Acid-fast staining technique. There are two different methods of stainging: 1) Ziehl-Neelsen Method and 2) Kinyoun Method.1) The Ziel-Neelsen method uses a primary stain of Carbol Fuchsin dye that must be steam treated, rinsed with acid alcohol wash, and a secondary stain of Methylene Blue.2) The Kinyoun Method uses a primary stain of Kinyoun Carbol Fuchsin dye that is not steam treated. An acid alcohol wash is applied and a secondary dye of Brilliant Green. This technique is called "cold staining".The mycolic acid within the Mycobacterium cell membrane has a high affinity for the Carbol Fuchsin dyes.
Where does Mycobacterium tuberculosis hide?
Mycobacterium tuberculosis does not hide; it simply has a coating that makes it hard to detect. This waxy coating on its cell surface makes the cell impervious to gram staining.
What is the Structure of mycobacterium tuberculosis in carbol fushin staining?
Mycobacterium tuberculosis is an obligate species in the family Mycobacterium and the causative agent of most cases of tuberculosis
What bacteria is used in acid-fast staining?
It is used for those bacteria which contain fat or lipid layer on their outer wall, and did not stain with grams staining. e.g. Mycobacterium
What are examples of acid-fast organisms?
Two acid-fast bacteria that are pathogenic are:1. Mycobacterium tuberculosis2. Mycobacterium avium- Microbioman
How might acid fast characteristic of mycobacterium enhance the organisms ability to cause disease?
Mycobacteria grow slowly, are protected from lysis once phagocytized, are capbable of intracellular growth, resistant to gram staining, many antimicrobial drugs, and from desiccation(drying out). mycobacteria are hard to treat and require a long treatment
Does the ability of a Mycobacterium tuberculosis cell to infect a new host depend on its drug-resistant phenotype?
No, drug-susceptible cells and drug-resistant cells are equally likely to infect a new host.
What relationship did you find if any between the Gram-staining reaction of a microorganism and its susceptibility to antiseptics and disinfectants?
Gram + bacteria has thick cell walls. This feature makes them more resistant to antiseptic and disinfectants.
What is fluorochrome staining technique for mycobacterium?
A flourochrome stain is an ultraviolet reactive dye that shines when exposed to UV. By using this stain, acid-fast bacilli (such as mycobacterium) can be easily seen. Flourochrome is used because standard techniques such as Gram stains can result in inconclusive results.
What would be an acid-fast bacteria?
Mycobacterium is am example for acid fast bacteria. These bacterias have large amounts of mycolic acids in their cell wall which are impermeable to any other staining technique.
How do you think the acid fast nature of mycobacterium contributes to it virulence?
Mycobacteria grow slowly, are protected from lysis once phagocytized, are capbable of intracellular growth, resistant to gram staining, many antimicrobial drugs, and from desiccation(drying out). mycobacteria are hard to treat and require a long treatment
What infections cannot be cured with antibotics?
Some infections are resistant or immune to antibiotics. These are not all created equal and are each immune to specific antibiotics. Important examples are methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus (VRE) and multi-drug-resistant Mycobacterium tuberculosis (MDR-TB) and Klebsiella pneumoniae carbapenemase-producing bacteria (KPC).
Why was Mycobacterium smegmatis used instead of Mycobacterium tuberculosis?
Mycobacterium smegmatis is a non pathogenic organism so it is used in labs to study about Mycobacterium tuberculosis .
