Because bacteria in this genus contain mycolic acid
the presence of mycolic acid
It has a peptidoglycan layer filled with mycolic acids. The acids make the cell wall waxy and impenetrable to stains. They are classified with gram positive cells because of cell wall thickness and genetic similarities.
Mycobacterium tuberculosis does not hide; it simply has a coating that makes it hard to detect. This waxy coating on its cell surface makes the cell impervious to gram staining.
it makes them water resistant
Gram + bacteria has thick cell walls. This feature makes them more resistant to antiseptic and disinfectants.
The acid-fast nature of mycobacterium contributes to its virulence because its waxy cell wall makes it hard for other cells to phagocytize it. The mycobacterium is therefore likely to live, reproduce and cause disease.
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Fixing the smear kills the bacteria and staining makes it more visible under the microscope.
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Some bacteria, particularly the mycobacterium, have cell walls which contain a wax-like lipid, mycolic acid, which makes it difficult for Gram staining reagents to penetrate the cells. For this reason, special stains and procedures are used. These stains penetrate the walls and retained even if the bacteria are treated with a strong decolorizing by acid alcohol and do not retain the carbolfuchsin stain are called non acid-fast.
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Desmosomes and tight junctions
The answer is Calcium.
Local ecosystem makes it possible for amphibians to resistant to BD. Amphibians cleared all infections.
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Staining is done to help determine what the sample is. It makes it visible under a microscope and it gives some information about the structure of the cell. A positive Gram stain means that the cell has a thick peptidoglycan layer.
Because it's made of clay
It kills the bacteria makes them adhere to the slide and coagulates their protein for better staining.