Is this an AP lab?
The DPIP replaces NADP+.
In photosynthesis, NADP is reduced to NADPH, but NADP is colorless.
DPIP replaces the NADP. When DPIP is reduced, it changes from blue to clear. DPIP is used to show photosynthesis taking place.
if you are referring to AP bio lab 4...
DPIP replaces or substitutes NADP+ molecules
It is the electron transporter
NADP+
If you are referring to the "Plant Pigments and Photosynthesis" Lab Then, the DPIP is used to substitute NADP+. In photosynthesis, electrons are normally transferred to NADP+. However, DPIP will take its place in this experiment. DPIP is normally blue. When it is reduced, or gains electrons, it will turn colorless. DPIP is used to show that photosynthesis is taking place.
NADP + would perform the same function. During photosynthesis electrons are transferred there.
Darkness means no reduction. NADP is in light reactions - DPIP is electron acceptor susbstitute for NADP... if NADP does not react in darkness (plants dont react at night and do not undergo photosynthesis) then neither should DPIP
The percent transmittance grew to steadily higher numbers as the experiment progressed because the light reaction was able to occur. However, the dark cuvettes had stable levels of transmittance because light is necessary to excite electrons, which, in turn, reduces the DPIP.
Boiling chloroplasts prevents the DPIP from being reduced because the enzymes for photosynthesis are no longer present in the chloroplasts. DPIP is reduced from blue to colorless when light strikes the chloroplasts and the electrons are boosted to a higher energy level. Since photosynthesis cannot be performed by the denatured chloroplasts, the DPIP cannot be reduced.
If you are referring to the "Plant Pigments and Photosynthesis" Lab Then, the DPIP is used to substitute NADP+. In photosynthesis, electrons are normally transferred to NADP+. However, DPIP will take its place in this experiment. DPIP is normally blue. When it is reduced, or gains electrons, it will turn colorless. DPIP is used to show that photosynthesis is taking place.
DPIP substitutes for electron acceptor
NADP + would perform the same function. During photosynthesis electrons are transferred there.
Because it was the control. Note how all other cuvettes had DPIP. We did not really know what dpip's effect on the % light transmittance was, by adding a ontrol, we could not compare and contrast.
In lab 4, DPIP is an electron acceptor that takes the place of NADP+. When DPIP is reduced, it changes from blue to colorless so you can see that the Calvin cycle is running.
Darkness means no reduction. NADP is in light reactions - DPIP is electron acceptor susbstitute for NADP... if NADP does not react in darkness (plants dont react at night and do not undergo photosynthesis) then neither should DPIP
the electrons came from splitting water molecules and were passed to the chlorophyll molecules, eventually being transferred to the DPIP
The percent transmittance grew to steadily higher numbers as the experiment progressed because the light reaction was able to occur. However, the dark cuvettes had stable levels of transmittance because light is necessary to excite electrons, which, in turn, reduces the DPIP.
Boiling chloroplasts prevents the DPIP from being reduced because the enzymes for photosynthesis are no longer present in the chloroplasts. DPIP is reduced from blue to colorless when light strikes the chloroplasts and the electrons are boosted to a higher energy level. Since photosynthesis cannot be performed by the denatured chloroplasts, the DPIP cannot be reduced.What_is_the_effect_of_boiling_chloroplasts_on_the_subsequent_reduction_of_DPIP
Boiling chloroplasts prevents the DPIP from being reduced because the enzymes for photosynthesis are no longer present in the chloroplasts. DPIP is reduced from blue to colorless when light strikes the chloroplasts and the electrons are boosted to a higher energy level. Since photosynthesis cannot be performed by the denatured chloroplasts, the DPIP cannot be reduced.
no answer for that question
It was an experiment in religious freedom and tolerance.