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benedict's test.
1.Take 8 to 12 test tubes and label them.. 2. Add 5 ml saline in each tube to provide isotonic medium.. 3.Add 5 cc blood sample in each tibe 4.Add 1 ml solution containing antibodies 5.Mix and shake gently 5.Took half of first test tube and add to 2nd tube then took half of 2nd tube and add to 3rd tube and son...It is serial dilution..Leave the last tube as such .dnt add antibodies in it..It is Control 6.Keep all test tubes in water bath at temp 37 deg C for 30 min 7.refrigerate them overnight at 4 deg C 8.Check agglutination in the tubes caused by Ag - Ab complex 9. Check the last tube having agglutination..Suppose its dilution is 1:32,then the titer of Ab will b 32.. BY:::dr sakhawat ali
To test starch: To test starch you take the food sample, and add iodine solution if the colour turns black this means starch is present. To test for protein: To test for protein, you take the food sample and add Biuret A and Biuret B and shake, if the colour turns lilac this means that protein is present.
Use Biuret reagent.
Add a sulfate solution: BaSO4 precipitates!
There is not a special test for negative ions IN GENERAL. However there are many possibillities for negative ions of each kind in particular: Example: test on Cl- : add silver nitrate: AgCl precipitate test on S2-: add drop of dilute acid: smell of rotten eggs (H2S) test on SO42-: add BaCl2 solution: BaSO4 precipitate
add 10mm each day
Reagent Blank : Take reagent and add deionised water (in place of sample to be tested). Now measure the OD at specific wavelength --> this OD is your reagent blank. Substract this OD from your test result (with sample) to avoid any false +ve effect due to colour of reagents itself.Sample Blank : Take sample and measure the OD without adding reagents --> this OD is your sample blank. Substract this OD from your test result to avoid any false +ve effect due to colour and turbidity of sample itself. As it is the fact that colour and turbidity of each sample would vary from one to another.So now it is clear that Reagent blank is used to avoid bias due to colour of reagents and Sample blank is used to avoid bias due to sample itself.
add vinegar
you do the observed-expected value and square it, then devide that by the expected you do this for each cell then you add them up also you can enter your data as a matrix on a calculator TI and go to stat, test, chi square test.
go to the local market that you get chlorine at and pool shock. buy test strips that test the water's chlorine, and just add an extra chlorine tablet each time you add chlorine. if its still really low and not even close to the amount you may want to add pool shock
in pcr technique we take original dna first heat it to separate to strands in thermocycler then add rna primer after the formation of about 10 sequences on both parental strands add dna polymerase to construct further
benedict's test.
They superscore, meaning they'll take the highest scores you get on each section and add them to get a composite total. Meaning, let's say you got these scores on each test: SAT Test I: Math (750), Writing (720), Reading (640) SAT Test II: Math (600), Writing (800), Reading (700) You add the highest score from each subject and boom, you go from a 2100 or 2110 to a 2250!
Biuret Test for presence of proteins:Principle:The test indicates the presence of peptide linkages(CO-NH) in proteins. The CO-NH groups in polypeptide chain form complex colour compounds with cupric hydroxide (Cu(OH)2) which is formed by the action of NaOH on CuSO4 .Reagents:1) 40% Sodium hydroxide(NaOH)2) 1% Copper sulphate(CuSO4)Procedure:In 3mL of sample solution add 3mL of 40% NaOH. To This mixture add few drops of 1% CuSO4 .Observation:Purple Violet or Pink colour develops.Inference:(CO-NH) linkages are present.
why should you add 8 drops of urine in the Benedicts test
it is a funnel having the shape of thistle flower n a long glass stem. this funnel is used to add reagents to reaction vessels. generally made of glass