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What traits of the E. coli were not altered in pGLO experiment?
Wiki User
∙ 13y ago
Colony shape and color
Wiki User
∙ 13y ago
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What was the purpose of examining the original pGLO solution with and without UV illumination?
The purpose of examining the original pGLO solution with UV illumination was to detect bacteria. The purpose of examining the original pGLO solution without UV illumination was to detect the possibility that DNA cells existed.
How do ampicillin resistant E. coli combat the lethal effects of the ampicillin antibiotic?
The pGlo plasmid contains an ampicillin resistance gene. This gene encodes an enzyme, β lactimase, which enzymatically degrades ampicillin. Therefore, bacteria that take up the plasmid (transformants) become resistant to ampicillin.
Which plates should be compared to prove that the changes that occurred were due to the procedure that you performed?
The best way to prove that these changes occurred in the transformation lab is to compare the control to the experimental plates. Cells that were not treated with the plasmit (LB/amp (-) pGLO and LB/amp/are (-) pGLO plates) could not grow on ampicillin, wheras cells that were treated with the plasmid (LB/amp (=) pGLO and lB/amp/ara (+) pGLO plate) can grow on the LB/amp plate. Thus, the plasmid must confer resistance to ampicillin.
What are the two possible sources of fluorescence within an Ecol colony when exposed to UV light?
pGLO and GFP
On which plate would you expect to find bacteria most like the original non transformed E. coli colonies you initially observed?
You would expect to find it in the plate labeled LB-, because that specific plate is the control plate, meaning it has nothing added to it, like the amipicilin or pFlouroGreen plasmid.
Bio ap ap pglo transformation?
What kind of question is that? I mean really genius. I mean all your saying is the labs name and the class...that is not a question.
Why use arabinose in the plate in pglo experiments?
The arabinose sugar binds to the RNA in the cell and helps turn on the gene expression to make GFP, green flourescent proteins. When you shine a UV light on the proteins, it will glow.
What is meant by a control plate in pglo experiments?
a control plate in your particular case is a ampicillin plate with no inoculation of your transformed bacteria. its purpose to make sure that during the process of handling the plate there were no contamination by bacteria in the surrounding.
Can you tell if these bcteria are ampicillin resistant by looking at them on the LB plate?
No. You cannot tell if the bacteria are ampicillin resistant just by looking at them. Both types of bacteria (those that are ampicillin resistant and those that are ampicillin sensitive) look similar when cultured, think about the colonies on the LB starter plate and the colonies on the +pGLO LB/amp plate.
What is the small ring of DNA in bacteria?
Bacteria generally have one (and only one) circular chromosome. However, they can also contain smaller rings of DNA called plasmids. These plasmids are generally not essential to cellular functions, but serve a variety of purposes. For example, they can serve as cloning vectors.
