Diphenylamine is a reagent for DNA.
DNA
DNA
organic
diphenylamine reagent
Blue color appears in diphenylamine test because of the reaction of nitrates.
Diphenylamine is a reagent for DNA.
diphenylamine binds with the deoxyribose molecule to form a blue complex
DNA
DNA
When adding diphenylamine to deoxyribose you will get a dark deep purple color. If you react diphenylamine with crude DNA you will get a pink-violet color. DIphenylamine test is quantitative and the darker the color the greater the concentration of DNA in the solution
organic
diphenylamine reagent
Mix 90ml. sulfuric acid with 10ml. water, to this add 0.5gm. diphenylamine
Pink Turns Blue was created in 1985.
it turns blue
One major difference between DNA and RNA is their sugar: DNA contains deoxyribose, whereas RNA contains ribose. This is what isdiphenylamine testfor deoxyribose for. In this process, the reaction between the Dische reagent and 2-deoxypentose results in the development of a blue color. The reaction depends on the conversion of the pentose to w-hydroxylaevulinic aldehyde which then reacts with diphenylamine to give a a blue colored complex. The intensity of the blue color is proportional to the concentration of DNA. Dische reagent does not react with the ribose sugar in RNA and does not form a blue-colored complex.Dische diphenylamine test-- A chemical test utilized to detect the presence of DNA in a substance.Mudit Mishra