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What type of light microscopy is used to visualize stained specimens?
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What staining procedures are needed to visualize the capsule of a bacterial cell?
The organisms are stained with fluorochromes, and when exposed to ultraviolet, violet, or blue light they become a bright image resulting from the fluorescent light emitted by them. This exposes the capsule.
Can you see the atoms of elements with a powerful school microscope?
No, you cannot see individual atoms of elements with a school microscope. Atoms are much smaller than the wavelength of visible light, so they cannot be resolved by optical microscopes. Specialized techniques such as scanning electron microscopy or atomic force microscopy are needed to visualize atoms.
Can we use the UV light in laminar air flow to see DNA bands in agarose gels stained with Ethidium bromide?
One cannot use the UV light installed in a laminar air flow hood to visualize DNA in an agarose gel. You will have to use an instrument called a UV transillumunator, which illuminates the gel from below to see the stained DNA.
Why are specimens sometimes stained?
we use sterile water because to avoid false result by microorganism having in the water and same reason for distilled also to avoid impurities like crystals of some metal ions and also to avoid other microorganisms
An advantage of fluorescence microscopy over staining techniques is that?
fluorescence microscopy can be used wit any light microscope
What is the differences between light reflection and light transmission microscopy?
Transmission microscopy and reflection microscopy refer to type of illumination used to view the object of interest in the microscope. Reflected light microscopy, also called episcopic illumination or just epi-illumination, uses top-down lighting to illuminate the specimen and the light is reflected back from the specimen to the viewer. This type of illumination is most often used with opaque specimens like metallurgical samples. Transmitted light microscopy, also called diascopic illumination, uses bottom-up illumination where the light is transmitted through the specimen to the viewer. This type of illumination is most often used with translucent specimens like biological cells. Detailed information about microscopes can be found at these links: Microscopy Primer - Florida State University Reflected Light Microscopy Optical Pathway - Java interactive image Transmitted Light Microscopy Optical Pathway - Java interactive image
Difference between basal lamina and basement membrane?
The term "basal lamina" is usually used with electron microscopy, while the term "basement membrane" is usually used with light microscopy. The structure known as the basement membrane in light microscopy refers to the stained structure anchoring an epithelial layer. This encompasses the basal lamina secreted by epithelial cells and typically a reticular lamina secreted by other cells.
Does light microscopy offer better magnification than electron microscopy?
No
What staining procedures are needed to visualize the capsule of a bacterial cell?
The organisms are stained with fluorochromes, and when exposed to ultraviolet, violet, or blue light they become a bright image resulting from the fluorescent light emitted by them. This exposes the capsule.
How are biopsy specimens handled?
Biopsy specimens are often sliced into thin slices, stained, mounted on a glass slide, and examined using a light microscope. Newer sample preparation techniques involve the rapid freezing of the sample and slicing of the still-frozen material.
Can you see the atoms of elements with a powerful school microscope?
No, you cannot see individual atoms of elements with a school microscope. Atoms are much smaller than the wavelength of visible light, so they cannot be resolved by optical microscopes. Specialized techniques such as scanning electron microscopy or atomic force microscopy are needed to visualize atoms.
Introduction in basic techniques in microscopy?
Introduction to basic techniques in microscopy involves light microscopy, laser scanning, types of dyes, the cell, electron microscopy, differential interface microscopy, histological stains and histochemical stains.
Can we use the UV light in laminar air flow to see DNA bands in agarose gels stained with Ethidium bromide?
One cannot use the UV light installed in a laminar air flow hood to visualize DNA in an agarose gel. You will have to use an instrument called a UV transillumunator, which illuminates the gel from below to see the stained DNA.
Why are specimens sometimes stained?
we use sterile water because to avoid false result by microorganism having in the water and same reason for distilled also to avoid impurities like crystals of some metal ions and also to avoid other microorganisms
An advantage of fluorescence microscopy over staining techniques is that?
fluorescence microscopy can be used wit any light microscope
What is the definition of the term fluorescence microscopy?
The term "fluorescence microscopy" is a type of light microscopy in which the specimen is irradiated at wavelengths that excite fluorochromes. In medicine, it is used to detect antigens.
What is Dark field microscopy?
Dark field microscopy (dark ground microscopy) describes microscopy methods, in both light and electron microscopy, which exclude the unscattered beam from the image. As a result, the field around the specimen (i.e. where there is no specimen to scatter the beam) is generally dark.
