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What will be the positions of genomic DNA plasmid DNA and RNA in the agarose gel electrophoresis?
Wiki User
∙ 11y ago
RNA band will be near the wells being single stranded,Genomic DNA will be at the centre of the gel being linear double stranded and plasmid DNA being circular moves faster and will be the brightest hence will be near the base..
Wiki User
∙ 11y ago
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Durning agarose electrophoresis of plasmid DNA why a smiling band does comes?
Smiling occurs because of uneven electric fields through the gel
Can plasmid DNA be used for 16srRNA amplification?
The 16s rRNA genes (rDNA) exist on genomic DNA. Therefore, plasmid has nothing to do with its amplification. However, if the 16s rRNA gene is cloned into the plasmid, it can be amplified.
What is the role of Ethidium bromide in plasmid isolation?
Ethidium bromide is an intercalator, meaning it inserts itself between the base pairs of DNA. Linear DNA pieces like the genomic DNA fragments bind more ethidium bromide than the circular plasmid DNA. The solution is placed into a tube that is spun extremely fast (roughly 50,000 revolutions per minute) in an ultracentrifuge for about a day. During this time the cesium chloride forms a gradient of lower density at the top of the tube and higher density at the bottom. The genomic and plasmid DNA form tight bands in this gradient. Since the plasmid DNA binds less ethidium bromide it is more dense and is located lower in the tube than the genomic DNA. RNA forms a separate band at the bottom of the tube. These three bands can be visualized by UV light.
When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
What is the difference between plasmid and episomes?
Plasmid DNA is an extra-chromosomal DNA molecule, it cannot link up with chromosomal DNA, and it contains the genetic informations that are necessary for its own replication. Episomes is any kind of extra-chromosomal DNA that can link up with chromosomal DNA. That is the main difference between them two. Episomes are usually larger than other extra-chromosomal DNA. An example of episome are the viruses, because they intergrate their genetic material into the host's chromosomal DNA.The only difference between the plasmid and the episome is the integration i-e plasmid can not integrate while episome can integrate into the genome.
Is agarose gel electrophoresis suitable for only gram negative plasmid DNA?
Agarose gel electrophoresis is suitable for ALL DNA.
Durning agarose electrophoresis of plasmid DNA why a smiling band does comes?
Smiling occurs because of uneven electric fields through the gel
Can plasmid DNA be used for 16srRNA amplification?
The 16s rRNA genes (rDNA) exist on genomic DNA. Therefore, plasmid has nothing to do with its amplification. However, if the 16s rRNA gene is cloned into the plasmid, it can be amplified.
You have propagated a vector in a non expression host and isolated the plasmid How can you confirm whether the plasmid that you got is exactly the vector which you propagated?
Check the size in agarose gel, extract it from the gel then purify it and grow it on selective plate.
What are some of the characteristics of cosmid?
A cosmid has the characteristics of a hybrid plasmid. They are used in the building of genomic libraries. They are ~200 base pairs long. More detailed information is available on Wikipedia.
What is the role of Ethidium bromide in plasmid isolation?
Ethidium bromide is an intercalator, meaning it inserts itself between the base pairs of DNA. Linear DNA pieces like the genomic DNA fragments bind more ethidium bromide than the circular plasmid DNA. The solution is placed into a tube that is spun extremely fast (roughly 50,000 revolutions per minute) in an ultracentrifuge for about a day. During this time the cesium chloride forms a gradient of lower density at the top of the tube and higher density at the bottom. The genomic and plasmid DNA form tight bands in this gradient. Since the plasmid DNA binds less ethidium bromide it is more dense and is located lower in the tube than the genomic DNA. RNA forms a separate band at the bottom of the tube. These three bands can be visualized by UV light.
What are BACs and YACs?
BAC = Bacterial Artificial Chromosome -200-300Kb -good for genomic libraries -uses the bacterial F (sex) plasmid -currently quite popular YAC = Yeast Artificial Chromosome -1Mb -good for genomic libraries also -difficult to manipulate and use -less popular
Circular extra genomic DNA is called?
A circular band of DNA that exists separate of chromosomal DNA is called a plasmid. Plasmids reside within bacteria cells and can replicate independently of the chromosomal DNA.
When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
What is the function of the Ti plasmid?
what is the function of the plasmid
Which is the plasmid that increases resistance to antibiotics?
R-plasmid
What plasmid that can degrade toluic acid?
TOL plasmid
